Identification of salt tolerance of Shanlan upland rice and screening of salt resistance index
Evaluating and screening of salt-tolerant rice germplasm materials is of a great scientific and practical significance to conserve and utilize Shanlan upland rice in Hainan.Potting experiments were made and 40 accessions of Shanlan upland rice,3 accessions of common upland rice and 3 accessions of common paddy rice were selected for treatment under salt stress at the germination stage and the whole growth stage.They were treated with 0.6%NaCl solution at the germination stage and 4g kg-1 at the whole growth stage.The 25 growth indexes related to the salt tolerance of rice were determined,and their salt tolerance at the whole growth stage was analyzed by using correlation analysis,grey relational analysis and comprehensive evaluation to screen salt tolerance indexes.The results showed that single plant yield,flag leaf area,total number of grains per panicle,seed set rate and effective panicle number varied significantly between rice accessions(P<0.05),and were highly sensitive to salt stress.Comprehensive evaluation of the salt tolerance of 46 accessions of rice at the whole growth stage showed that 46 accessions can be divided into 4 categories:9 accessions with high salt tolerance that could complete the growth period under salt stress;3 accessions with high salt tolerance that could enter the reproductive growth stage but cannot form yield;16 accessions with general salt tolerance that died before entering the reproductive growth;18 accessions with poor salt tolerance that died after 35 days of salt stress.Through principal component analysis and membership function analysis,the comprehensive measurement value(D value)of salt resistance was obtained,and the Shanlan upland rice'WMS02'and the common upland rice'Huangyedao'were selected as materials with high salt resistance.Correlation analysis and grey relational analysis showed the penultimate leaf area and plant height at the tillering stage were identified as effective indexes for the identification of early stage salt tolerance.
upland riceShanlan upland ricefull growth periodsalt toleranceevaluating index
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