甘蔗环斑病致病菌的鉴定及生物学特性分析
Pathogen identification and biological characterization of sugarcane ring spot disease
谢蒙潇 1丘舒琪 1张怡 1谭丽花 1刘月廉 1胡汉桥 2王冠 3任磊 2王燕燕1
作者信息
- 1. 广东海洋大学 滨海农业学院,广东 湛江 524088
- 2. 广东海洋大学 滨海农业学院,广东 湛江 524088;国家耐盐碱水稻技术创新中心华南分中心,广东 湛江 524088
- 3. 海南农乐南繁科技有限公司,海南 三亚 572000
- 折叠
摘要
甘蔗环斑病是甘蔗常见的一种病害,在我国广东、广西等地较为常见.本研究从广西玉林地区的甘蔗病叶中分离到1株真菌菌株,命名为YLES 1,并按照科赫氏法则对分离菌进行致病性测定,证实了分离菌是甘蔗环斑病的病原菌.通过病原菌的形态观察,发现该真菌与附球菌属(Epicoccum)的形态特征类似.基于分离菌株的核糖体DNA转录间隔区(ITS)、大亚基r DNA(LSU)及β-微管蛋白(Tu)基因序列构建多基因联合系统发育树,其中确定YLES 1属于E.sorghinum,是甘蔗环斑病的病原物.该菌株的生物特性分析结果显示,YLES 1菌株生长的最适温度为28℃,菌丝生长和产孢的最适pH为7～9,最适生长和产孢碳源为蔗糖,最适氮源为酵母浸粉,且全黑暗的条件有利于菌YLES 1的生长.
Abstract
Sugarcane ring spot is a common disease in sugarcane,which is more common in Guangdong and Guangxi,China.One fungal strain,YLES 1,was isolated from sugarcane diseased leaves in the regions of Guangxi province.The pathogenicity of this strain was determined according to Koch's postulates,confirming that it is a pathogen causing sugarcane ring spot disease.This fungus has similar morphological characteristics to Epicoccum.Sequence analyses based on concatenated data sets of the internal transcribed spacer(ITS)region of the ribosomal DNA(rDNA),large subunit rDNA(LSU)and β-tubulin(Tu)of the isolate showed that the strain YLES 1 was E.sorginum.The results indicated that E.sorginum was a pathogen of sugarcane ring spot disease.The biological characteristic analysis showed that the optimal temperature for the growth of the strain YLES 1was 28℃,that the optimal pH for mycelial growth and spore production was 7-9,that the optimal carbon source for growth and spore production was sucrose,and that the optimal nitrogen source was yeast extract.Moreover,dark conditions were conducive to the growth of YLES 1.
关键词
甘蔗/环斑病/附球菌属/生物学特性Key words
sugarcane/ring spot disease/Epicoccum/biological characteristics引用本文复制引用
基金项目
广东海洋大学科研启动经费(060302052312)
南海青年学者项目(002029002004)
大学生创新创业训练项目(010403052332)
出版年
2024
NETL
NSTL
万方数据