Pathogen identification and biological characterization of sugarcane ring spot disease
Sugarcane ring spot is a common disease in sugarcane,which is more common in Guangdong and Guangxi,China.One fungal strain,YLES 1,was isolated from sugarcane diseased leaves in the regions of Guangxi province.The pathogenicity of this strain was determined according to Koch's postulates,confirming that it is a pathogen causing sugarcane ring spot disease.This fungus has similar morphological characteristics to Epicoccum.Sequence analyses based on concatenated data sets of the internal transcribed spacer(ITS)region of the ribosomal DNA(rDNA),large subunit rDNA(LSU)and β-tubulin(Tu)of the isolate showed that the strain YLES 1 was E.sorginum.The results indicated that E.sorginum was a pathogen of sugarcane ring spot disease.The biological characteristic analysis showed that the optimal temperature for the growth of the strain YLES 1was 28℃,that the optimal pH for mycelial growth and spore production was 7-9,that the optimal carbon source for growth and spore production was sucrose,and that the optimal nitrogen source was yeast extract.Moreover,dark conditions were conducive to the growth of YLES 1.
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