Cloning and expression analysis of visual genes of Megalurothrips usitatus
Megalurothrips usitatus is an important thrips pest of legume crops,with obvious phototaxis(color),and the light environment can affect its behavior and physiology.Four visual genes were cloned and their sequence data were uploaded to NCBI for searching.Moreover,the expression changes of the visual genes at different ages and genders were detected by real-time PCR.The results indicate that all four visual genes belong to the G protein coupled receptor family members.There were significant differences in expression levels of four visual genes among different age groups of M.usitatus(P<0.05),and their expression levels fluctuated in different age groups.There is a significant difference in the expression level of visual genes between female and male adults(P<0.05),and the expression level of visual genes in males is higher than that in females at all ages.This study clarified the molecular characteristics of the visual genes of M.usitatus and revealed the patterns of changes during their development,which has important guiding significance for precision control of thrips in the field using methods such as blue sticky insect plates or changing the light environment.
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万方数据
