Cloning,sequences analysis and phylogenetic tree construction
In order to explore the structure and function of koi sox10b and its amino acids,the coding se-quence(CDS)of koi sox10b was cloned by RT-PCR.Then,the multiple sequences alignment and phylogenetic tree of the amino acid sequences of sox10 among 13 species were conducted.The results showed that the length of koi sox10b CDS was 1452bp,encoding 483 amino acids.Amino acid sequences alignment result showed that koi Sox10b had conserved Sox_N Superfamily domain and HMG-box_SoxE Superfamily domain.The phylogenetic tree showed that koi sox10b was closely related to the common carp sox10b and zebrafish sox10,which suggest the koi sox10b may perform similar functions with zebrafish sox10.
NETL
NSTL
万方数据
