Study on Improving Hydrolysis Efficiency of Corn Stover by the Combined Pretreatments of Ball Milling and Steam
To develop an efficient and environmentally friendly lignocellulosic pretreatment method,we utilized corn stover(CS)as a model material and proposed a combination of ball milling and steam pretreatments to enhance the CS digestibility.Our results demonstrated that the ball milling pretreatment of CS with 4 mm in diame-ter markedly improved its digestibility,increasing glucose and TRS recoveries from 38% and 36% to 65% and 58% at a solid loading of 10%,respectively.Additionally,100%recoveries of both glucose and TRS after enzymatic hy-drolysis were achieved when the CS pretreated by the ball milling was further subjected to the steam pretreatment at 121 ℃ for 1 hour after immersion in 6 g·L-1 NaOH.Even at a solid loading of 30%,the glucose and TRS recover-ies remained high,reaching 77.14% and 75.82%,respectively,under these pretreatment conditions.However,TOC and TN analyses indicated a significant increase in the digestibility of non-sugar byproducts with the CS digest-ibility increasing,which had downstream impacts on the lipid fermentation process.Rhodosporidium toruloides could grow and produce lipid in the CS hydrolysate at a solid loading of 20%,with the final biomass,lipid and lipid con-tent being 16.00 g·L-1,6.00 g·L-1 and 35.61%,respectively.
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