球磨与蒸汽联合处理玉米秸秆提高水解效率的研究
Study on Improving Hydrolysis Efficiency of Corn Stover by the Combined Pretreatments of Ball Milling and Steam
黄其田 1RASOOL Kamal 1陆洪斌 1张珺璐 2龚志伟 2赵宗保1
作者信息
- 1. 中国科学院大连化学物理研究所生物技术部,中国 大连 116023
- 2. 武汉科技大学化学与化工学院,中国 武汉 430080
- 折叠
摘要
为了开发绿色且高效的木质纤维素处理新技术,以玉米秸秆为模型原料,将球磨预处理与蒸汽预处理相结合用以提高玉米秸秆水解效率.研究结果表明:球磨预处理可显著提高秸秆水解效率,在 10%固载量条件下,4mm秸秆经过球磨预处理后,水解后葡萄糖和还原糖的回收率分别由38%和36%提高至65%和58%;在球磨预处理的基础上,将秸秆浸入 6 g·L-1 NaOH并在 121℃条件下蒸汽预处理 1h,水解后葡萄糖和还原糖的回收率均达到100%,且在此预处理条件下,当固载量由 10%增加至 30%时,葡萄糖和还原糖的回收率仍可达到 77.14%和75.82%;另外,TOC和TN分析表明,在预处理条件优化和水解效率显著提高的同时,秸秆中的非糖副产物的水解率也显著提高,圆红酵母最高可在秸秆固载量为 20%时获得的水解液中进行生长和油脂发酵,生物量、油脂产量和油脂含量分别为 16.00 g·L-1,6.00 g·L-1和 35.61%.
Abstract
To develop an efficient and environmentally friendly lignocellulosic pretreatment method,we utilized corn stover(CS)as a model material and proposed a combination of ball milling and steam pretreatments to enhance the CS digestibility.Our results demonstrated that the ball milling pretreatment of CS with 4 mm in diame-ter markedly improved its digestibility,increasing glucose and TRS recoveries from 38% and 36% to 65% and 58% at a solid loading of 10%,respectively.Additionally,100%recoveries of both glucose and TRS after enzymatic hy-drolysis were achieved when the CS pretreated by the ball milling was further subjected to the steam pretreatment at 121 ℃ for 1 hour after immersion in 6 g·L-1 NaOH.Even at a solid loading of 30%,the glucose and TRS recover-ies remained high,reaching 77.14% and 75.82%,respectively,under these pretreatment conditions.However,TOC and TN analyses indicated a significant increase in the digestibility of non-sugar byproducts with the CS digest-ibility increasing,which had downstream impacts on the lipid fermentation process.Rhodosporidium toruloides could grow and produce lipid in the CS hydrolysate at a solid loading of 20%,with the final biomass,lipid and lipid con-tent being 16.00 g·L-1,6.00 g·L-1 and 35.61%,respectively.
关键词
木质纤维素/预处理/酶法水解/圆红酵母/油脂Key words
lignocellulose/pretreatment/enzymatic hydrolysis/Rhodosporidium toruloides/lipid引用本文复制引用
基金项目
国家重点研发计划(2021YFC2103702)
国家自然科学基金重点项目(22238010)
国家自然科学基金青年项目(22208340)
出版年
2024
NETL
NSTL
万方数据