In order to investigate the effect of usnic acid on human bladder cancer,cultured human bladder cancer cells T24 and RT4 were treated with different concentrations of usnic acid(12.5,25,50,100,200 μmol/L)for 24 h and 48 h,respectively,and the proliferation of T24 and RT4 cells was detected by MTT assay.The re-sults showed that intracellular reactive oxygen species(ROS)levels were increased after treatment with usnic acid,and apoptosis of T24 cells was induced through mitochondrial pathway and death receptor pathway:(1)Usnic acid inhibited the proliferation of T24 and RT4 cells in a time-dependent and dose-dependent manner,and the inhibitory rate of usnic acid on T24 cell proliferation was higher.(2)By Hoechst 33342 staining and An-nexin V-FITC/PI double staining,apoptotic T24 cells gradually increased with the increase of the concentration of usnic acid.(3)After treatment with usnic acid,the level of intracellular ROS increased,the expression of Bax,cleaved-Caspase-8,cleaved-Caspase-9 and cleaved-Caspase-3 protein increased,the expression of Bcl-2,Caspase-3,Caspase-8 and caspase-9 decreased,and the ratio of Bax protein expression to Bcl-2 pro-tein expression increased.
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