松萝酸抑制人膀胱癌细胞增殖作用机制研究
Inhibitory Effect of Usnic Acid on Proliferation of Human Bladder Cancer Cells
曹鹤 1史丽颖 1郭秀磊 1于大永1
作者信息
- 1. 大连大学生命科学与技术学院,大连 116622
- 折叠
摘要
为了探究松萝酸对人膀胱癌的影响,体外培养人膀胱癌细胞T24、RT4,用不同浓度(12.5、25、50、100、200μmol/L)的松萝酸分别处理T24、RT4细胞24 h和48 h,采用MTT法检测T24、RT4细胞的增殖情况.研究结果发现松萝酸处理后细胞内的活性氧(ROS)水平增加且通过线粒体途径、死亡受体途径来诱导T24细胞发生凋亡:(1)松萝酸对T24、RT4细胞增殖均有抑制作用,且均呈现时间和剂量依赖性,其中,松萝酸对T24细胞增殖的抑制率更高;(2)通过Hoechst 33342染色与Annexin V-FITC/PI双染发现,随着松萝酸浓度的升高,凋亡的T24细胞逐渐增多;(3)T24 细胞经松萝酸处理后,细胞内 ROS 水平升高,cleaved-Caspase-3、cleaved-Caspase-8、cleaved-Caspase-9 和 Bax蛋白的表达量增加,Caspase-3、Caspase-8、Caspase-9和Bcl-2蛋白的表达量降低,且Bax蛋白表达量与Bel-2蛋白表达量的比值增加.
Abstract
In order to investigate the effect of usnic acid on human bladder cancer,cultured human bladder cancer cells T24 and RT4 were treated with different concentrations of usnic acid(12.5,25,50,100,200 μmol/L)for 24 h and 48 h,respectively,and the proliferation of T24 and RT4 cells was detected by MTT assay.The re-sults showed that intracellular reactive oxygen species(ROS)levels were increased after treatment with usnic acid,and apoptosis of T24 cells was induced through mitochondrial pathway and death receptor pathway:(1)Usnic acid inhibited the proliferation of T24 and RT4 cells in a time-dependent and dose-dependent manner,and the inhibitory rate of usnic acid on T24 cell proliferation was higher.(2)By Hoechst 33342 staining and An-nexin V-FITC/PI double staining,apoptotic T24 cells gradually increased with the increase of the concentration of usnic acid.(3)After treatment with usnic acid,the level of intracellular ROS increased,the expression of Bax,cleaved-Caspase-8,cleaved-Caspase-9 and cleaved-Caspase-3 protein increased,the expression of Bcl-2,Caspase-3,Caspase-8 and caspase-9 decreased,and the ratio of Bax protein expression to Bcl-2 pro-tein expression increased.
关键词
松萝酸/膀胱癌/凋亡/线粒体途径/死亡受体途径Key words
usnic acid/bladder cancer/apoptosis/mitochondrial pathway/death receptor pathway引用本文复制引用
基金项目
辽宁省科学技术计划项目(2019-ZD-0564)
出版年
2024
NETL
NSTL
维普
万方数据