Rapid determination of five poppy shell alkaloids in Liangpi soup seasoning by ultra performance liquid chromatography-tandem mass spectrometry
Objective To establish a method for the rapid determination of five poppy shell alkaloids in Liangpi soup seasoning using ultra-high-performance liquid chromatography-tandem mass spectrometry.Methods The accurately weighed sample was mixed with acetonitrile,and after vortex mixing,anhydrous sodium acetate and anhydrous magnesium sulfate were added for extraction and centrifugation.The supernatant was purified by ethylenediamine-N-propylsilane powder and separated by HILIC column,and then determined by multiple reaction monitoring mode and external standard method.Results The linear relationship was good in the range of 0.05-2.0 ng/mL for papaverine,noscapine,and thebaine and in the range of 0.25-10.0 ng/mL for morphine and codeine,The correlation coefficients were all greater than 0.999 0.The detection limits were 0.03 μg/kg for papaverine,noscapine,and thebaine,and 0.15 μg/kg for morphine and codeine.The spiked recovery rates were between 81.3％and 98.6％,and the relative standard deviations were between 2.1％and 4.9％.Conclusions With simple and efficient pretreatment,this method is economic,scientific,sensitive and accurate,and suitable for the rapid and accurate determination of alkaloids from poppy shells in batch Liangpi soup seasoning.
Ultra-high-performance liquid chromatography-tandem mass spectrometryPoppy shellAlkaloidLiangpi soup seasoning
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