Objective To explore the impact of peripheral blood sample storage temperature and duration on the quality of chromosome G banding karyotype preparation.Methods The indexes of number of nucleated cells,mitotic index and number of mitotic phase cells were used to assess the quality of preparation with the samples stored at 4 ℃ and RT(room temperature,18-22℃)for various time durations(0,3,5,7 days),respectively.Results The number of nucleated cells harvested decreased with increasing storage duration at 4 ℃ or RT conditions.The mitotic index did not decrease significantly for samples stored at 4 ℃within 7 days and at RT within 3 days[6.2(1.8)％vs 7.2(1.9)％,8.2(2.0)％vs 7.2(1.9)％,both P＞0.05];the samples stored at 4 ℃for 3 days showed a slight increase in the mitotic index,but the difference was not significant[9.7(2.9)％vs 7.2(1.9)％,P＞0.05].Storing samples at 4 ℃ within 7 days or at RT within 3 days were still abel to provide sufficient mitotic phase cells for karyotype analysis.Additionally,the difference in mitotic phase cells between samples stored at 4 ℃ for up to 3 days and fresh samples was not statistically significant[10.7(3.2)x 104 vs 11.4(3.5)x104,P＞0.05].Conclusions Peripheral blood samples stored at 4 ℃within 7 days or at RT within 3 days can be used for chromosome preparation.
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