Impact of peripheral blood sample storage temperature and duration on the chromosomal preparation
Objective To explore the impact of peripheral blood sample storage temperature and duration on the quality of chromosome G banding karyotype preparation.Methods The indexes of number of nucleated cells,mitotic index and number of mitotic phase cells were used to assess the quality of preparation with the samples stored at 4 ℃ and RT(room temperature,18-22℃)for various time durations(0,3,5,7 days),respectively.Results The number of nucleated cells harvested decreased with increasing storage duration at 4 ℃ or RT conditions.The mitotic index did not decrease significantly for samples stored at 4 ℃within 7 days and at RT within 3 days[6.2(1.8)％vs 7.2(1.9)％,8.2(2.0)％vs 7.2(1.9)％,both P＞0.05];the samples stored at 4 ℃for 3 days showed a slight increase in the mitotic index,but the difference was not significant[9.7(2.9)％vs 7.2(1.9)％,P＞0.05].Storing samples at 4 ℃ within 7 days or at RT within 3 days were still abel to provide sufficient mitotic phase cells for karyotype analysis.Additionally,the difference in mitotic phase cells between samples stored at 4 ℃ for up to 3 days and fresh samples was not statistically significant[10.7(3.2)x 104 vs 11.4(3.5)x104,P＞0.05].Conclusions Peripheral blood samples stored at 4 ℃within 7 days or at RT within 3 days can be used for chromosome preparation.
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