Monitoring results of SARS-CoV-2 in urban sewage in Zhengzhou city
Objective To analyze the monitoring results of SARS-CoV-2 in environmental sewage in Zhengzhou city,Henan province,provide data support for environmental monitoring,and preliminarily explore the role of environmental monitoring in COVID-19 prevention and control.Methods Two sewage treatment plants in Zhengzhou city were selected as monitoring sites.From March-July 2023 each sewage treatment plant collected two samples of sewage per week;the enrichment was conducted by PEG method.Multiple real-time fluorescence reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect and analyze novel coronavirus;the whole genome sequence was obtained by SARS-CoV-2 sequencing,and the virus type was judged by analysis software.Results The total detection rate of novel coronavirus in urban sewage was 89.09％.From March to April,the detection rate of novel coronavirus was 76.47％and 81.82％respectively,and from May to July,the detection rate was 100.00％.Of the 55 sewage samples collected,SARS-CoV-2 was detected in sewage solution,sediment,supernatant and concentrated samples.Virus content size:concentrated sample＞stock solution＞supernatant＞sediment.N gene calculation showed that the concentration of SARS-CoV-2 in concentrated samples was 13.57 times that in sewage solution.The concentration of SARS-CoV-2 in urban sewage peaked in the 23rd week,then began to decline,and then dropped to a low point in the 26th week.In May,the top two viruses in sewage were XBB.1.9,XBB.1.16 and its sub-branches.Conclusions The detection rate of SARS-CoV-2 in environmental sewage in Zhengzhou is high,especially from May to July.The virus type is Omicron XBB subbranch,and environmental monitoring can be used as a supplement to hospital case monitoring.
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