Pathogenic surveillance results of hand,foot and mouth disease and characteristics of VP1 gene of Coxsackievirus A6,in Liuzhou city,Guangxi Zhuang Autonomous Region,2019-2022
Objective To describe the pathogenic surveillance results of hand,foot and mouth disease(HFMD)in Liuzhou from 2019 to 2022,analyze the characteristics of VP1 gene of Coxsackievirus A6(CoxA6)identified,and capture the pathogenic composition of HFMD and the molecular epidemiological characteristics of CoxA6 in the region.Methods Collect anal swab,pharyngeal swab,and herpes fluid samples from children diagnosed with HFMD at Liuzhou city Hand,Foot and Mouth Disease Monitoring Outpost Hospital from 2019 to 2022.Quantitative fluorescent PCR was used for the pathogen detection and typing;VP1 gene of CoxA6 isolates identified from some severe cases was sequenced and analyzed.Results From 2019 to 2022,a total of 1 432 samples from HFMD cases were collected in Liuzhou city,and 1 224 were tested positive,with a positive rate of 85.47％.Of them,547(44.69％)were positive for CoxA6;428(34.97％)were positive for CoxA16;22(1.80％)were positive for EV71;60(4.90％)were positive for CoxA10 and 167(13.64％)were positive for other enteroviruses.The six CoxA6 isolates that caused severe HFMD in Liuzhou city in 2019 mainly included three strains,which were closely related to the strains identified in Guangdong in 2017,in Shanghai in 2018 and in Jiangxi in 2019,respectively.Conclusions CoxA6,CoxA16,CoxA10 and other enteroviruses are the main pathogens of HFMD in Liuzhou city from 2019 to 2022,and CoxA6 has become the dominant pathogen in Liuzhou city.The nucleotide homology of VP1 gene was from 93.5％to 100.0％,and the amino acid homology was from 98.3％to 100.0％,both belonging to the D3 gene subtype of D genotype.Pathogen monitoring should be further strengthened to provide a basis for implementing comprehensive prevention and control measures of HFMD.
Hand,foot and mouth diseaseEtiologyCoxsackievirus A6Genetic characteristics
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NSTL
万方数据
