Comparison of the second retest and complementary tests results of HIV-1 antibody screening reactive samples in a city,Henan province
Objective To analyze the second retest and complementary tests(nucleic acid test and antibody confirmation test)results of HIV-1 antibody screening reactive samples,and provide the evidence for HIV-1 detection strategy.Methods From February 2022 to February 2023,141 HIV-1 antibody screening reactive samples sent by medical institutions were tested for antibody second retest,viral load(VL),and antibody confirmation test by Western blot(WB).Results Among the 141 HIV-1 antibody screening reactive samples,131(92.91％)samples had response,and 10(7.09％)samples had no response in the second retest.The positive rate in the second retest was 50％(1/2)for pregnant women,91.67％(77/84)for preoperative examination,94.74％(36/38)for voluntary consultation,100.00％(5/5)for physical examination(marriage examination,recruitment examination and normal physical examination),and 100.00％(7/7)for other persons(detainees and previous confirmed patients).HIV viral load detection results were＜50 copies/mL for 13(9.92％)samples,between 50-＜1 000 copies/mL for 8(6.11％)samples,between 1 000-5 000 copies/mL for 13(9.92％)samples,and ≥5 000 copies/mL for 97(74.05％)samples.Of the 21 samples with VL detection results between 50-5 000 copies/mL,WB results were all HIV-1 antibody positive,and 9 band types were found.They were mainly full band.There were no bands in 10 samples of no response after the second retest,and VL detection results were all＜50 copies/mL.Conclusions It can improve the level of HIV detection rate and quickly lock the source of infection to reduce the HIV-1 viral load positive threshold and use antibody retest and nucleic acid detection.
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