北京市昌平区副溶血性弧菌临床分离株病原学特征与耐药分析
Analysis on pathogenic characteristics and drug resistance of Vibrio Parahaemolyticus strains isolated from clinical specimens in Changping district of Beijing
张阳 1李东迅 1王维钧 1彭华 1银安琪 1舒高林1
作者信息
- 1. 北京市昌平区疾病预防控制中心,北京 102206
- 折叠
摘要
目的 了解北京市昌平区副溶血性弧菌血清型分布、分子流行病学特征、毒力基因携带情况等病原学特征及耐药性.方法 对2015-2019年北京市昌平区分离到的134株副溶血性弧菌进行血清分型、多位点序列分型(MLST)、3种毒力基因(tlh、tdh、trh)荧光PCR检测和12种抗生素耐药性分析.结果 134株菌共分为13个血清型,以O3:K6血清型为主(73.88％);共鉴定出31个ST型,以ST655(59.70％)为主;共分为两种毒力基因谱,以tlh+tdh+trh-(94.78％)为主;对头孢唑啉耐药率(97.01％)最高,其次为氨苄西林(56.72％).结论 北京市昌平区引起腹泻的副溶血性弧菌有较严重的耐药性,血清型以O3:K6为主,ST型以ST655为主,多数菌株毒力基因为tlh+tdh+trh-.
Abstract
Objective To understand the pathogenic characteristics including serotype distribution,molecular characteristic and virulence genes,and drug resistance of Vibrio Parahaemolyticus strains in Changping district of Beijing.Methods In total,134 strains of Vibrio Parahaemolyticus isolated in Changping district of Beijing between 2015 and 2019 were analyzed by serotyping,multilocus sequence typing(MLST),tlh/tdh/trh virulence genes detection and antimicrobial susceptibility testing of 12 antibiotics.Results Among the 134 Vibrio Parahaemolyticus strains,13 serotypes were detected,and O3:K6(73.88％)was the dominant serotype.A total of 31 sequence types(STs)were assigned,and the predominant STs were ST655(59.70％).Two virulence genes profiles were found,and the dominant virulence genes profile was tlh+tdh+trh-(94.78％).The resistance rate of cefazolin(97.01％)was highest,followed by ampicillin(56.72％).Conclusions Vibrio Parahaemolyticus strains causing diarrhea has severe drug resistance in Changping district,Beijing.The dominant serotype was O3:K6,and the predominant STs was ST655.Most strains had the virulence gene profile tlh+tdh+trh-.
关键词
副溶血性弧菌/血清型/多位点序列分型/耐药性/毒力基因Key words
Vibrio parahaemolyticus/Serotypes/Multilocus sequence typing/Drug resistance/Virulence genes引用本文复制引用
出版年
2024
NETL
NSTL
万方数据