Genetic characterization of influenza A(H3N2)virus in Anyang City,Henan Province in 2022
Objective To analyze the genetic characteristics of influenza A(H3N2)virus in Anyang City,Henan Province in 2022,so as to provide an evidence for scientific prevention and control of influenza.Methods The whole genome sequence of 17 influenza A(H3N2)viruses in Anyang City in 2022 was determined by sequencing technology;HA and NA gene sequences of influenza A(H3N2)viruses were compared by bioinformatics software;the molecular phylogenetic tree was constructed and the genetic characteristics were analyzed.Results The nucleotide homology of the 17 influenza virus isolates in Anyang City was from 98.7％to 100.0％,and the homology of amino acid sequences was from 98.1％to 100.0％.Compared with the vaccine strain A/Cambodia/e0826360/2020(H3N2),the homology of nucleotide sequence was from 95.7％-99.4％,and the homology of amino acid sequence was from 96.9％-98.6％.The 17 influenza A H3N2 virus isolates all belonged to the 3C.2a1b.2a.1a.1 clade,and compared with the vaccine strain A/Cambodia/e0826360/2020(H3N2),there were 16 amino acid site mutations in HA gene,among which none of the amino acid mutation sites were in the HA antigenic determinant cluster and RBS.A total of 13 amino acid sites were mutated in the NA gene,different from the currently reported mutation sites related to drug resistance.The number and location of potential N-glycosylation sites in HA and NA all changed.Conclusions In 2022,the influenza A(H3N2)virus in Anyang City did not mutate significantly during the epidemic.However,the mutation of multiple amino acid sites in HA and NA suggests that dynamic monitoring of influenza A(H3N2)virus at the molecular level still needs to be strengthened.
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