Improvement of the graphite furnace atomic absorption spectroscopy method for the determination of blood lead
Objective To improve the method of acid-deproteinization graphite furnace atomic absorption spectroscopy for determining lead in human blood.Methods After deproteinizing the blood sample with acid,the 1.00 g/100 mL ammonium dihydrogen phosphate solution was used as a matrix modifier to optimize the graphite furnace heating program.The Zeeman 2 magnetic field background correction method was used,and an automatic sampler was used to prepare the standard series.The instrument automatically deducted the reagent blank and measured the absorbance using a graphite furnace atomic absorption spectrophotometer at the wavelength of 283.3 nm.Results This method had good linearity in the range of 50.0-500.0 μg/L(correlation coefficient r=0.999),and the relative standard deviation(RSD)of different concentrations of blood lead was between 1.08％-2.08％.The detection results of standard substances at different concentrations were within the allowable deviation range,and the recovery rate of spiked samples was between 104.9％-106.8％.Conclusions The improved acid-deproteinization graphite furnace atomic absorption spectroscopy method for the determination of lead in blood has simple operation,low interference,and higher precision than the national standard method.Its accuracy meets the national regulations,and it is suitable for the determination of lead content in blood samples.
NETL
NSTL
万方数据
