Assessment of diarrheagnic Escherichia coli contamination and virulence gene in food in a city of Henan Province,2019-2023
Objective To investigate the prevalence and distribution of virulence genes in diarrheagnic Escherichia coli(DEC)in food samples from a city in Henan Province,so as to provide data to inform the development of foodborne disease prevention and control strategies.Methods Following Guidelines of the Handbook of Monitoring Microbes and Their Pathogenic Factors in Food in Henan Province,a total of 339 food samples collected from 2019 to 2023 were analyzed.DEC strains were isolated,identified,and genotyped the virulence genes.Results The overall detection rate of DEC in food samples was 9.7％(33/339).Statistically significant variations were observed in detection rates across different years(x2=12.626,P＜0.05).The detection rates among different food categories were statistically significant(x2=30.795,P＜0.05)with the highest detection rate in fresh meat samples(20.4％).Bulk food samples had a higher detection rate compared to packaged food(x2=29.364,P＜0.05).Thirty-six virulent DEC strains were isolated and characterized,with enteroaggregative Escherichia coli(AECE)was the dominant strain.The primary virulence genes identified were the aggregative heat-stable toxin A gene and intestinal colonization factor gene.Conclusion Food in the studied city is significantly contaminated with DEC,with AECE is the dominant virulence strain of food contamination.
NETL
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万方数据
