Network Pharmacology and Bioinformatics Study on the Treatment of Lumbar Intervertebral Disc Degeneration by Zhuijianpan Pill
Objective To investigate the main active ingredients and molecular mechanism of Zhui-jianpan pill(ZJPW)in the treatment of lumbar intervertebral disc degeneration.Methods The main active ingredients and targets of ZJPW were obtained from TCMSP database,and the targets of lumbar in-tervertebral disc degeneration were screened from GeneCards database,TTD database,OMIM database and GEO database.Cytoscape 3.9.1 software was used to construct"drug-component-target"networks and analyze gene ontology(GO)functions and Kyoto Encyclopedia of Genes and Genomes(KEGG)en-richment of disease target genes.PPI network was constructed to screen out the main pathogenic genes of lumbar intervertebral disc degeneration,and molecular docking technology was used to simulate the inter-action between drug active ingredients and disease targets.Finally,immune infiltration analysis was per-formed using the CIBERSORT tool.Results A total of 233 active ingredients of ZJPW were obtained,and the main active components included quercetin,kaempferol,and hansenin.A total of 1,283 targets of ZJPW were identified,of which 23 were closely related to lumbar disc degeneration.These target genes were mainly enriched in the PI3K-Akt signaling pathway and the signaling pathway related to hu-man papillomavirus infection.The main pathogenic targets of lumbar disc degeneration were screened by PPI network as PTGS2,HIF1A,VEGFA,FN1 and CAV1.Molecular docking simulations revealed that the main active ingredients of ZJPW could bind to these main pathogenic targets of lumbar disc degenera-tion with low binding energy.Immune infiltration analysis revealed a significant positive correlation be-tween HIF1A and NK cell activation.Conclusion The active components of ZJPW may act at different stages of lumbar disc degeneration by modulating the PI3K-Akt signaling pathway through PTGS2,HIF1A,VEGFA,FN1 and CAV1.Among them,the HIF1A/NK cell axis may be one of the important links in the inhibition of disc degeneration by ZJPW.
万方数据
维普
