Study on the Mechanism of Dampness-Resolving and Pain-Relieving Decoction in the Treatment of Knee Osteoarthritis in Rats Based on Serum Metabonomics
Objective:To study the mechanism of Dampness-Resolving and Pain-Relieving Decoction on Knee Osteoarthritis(KOA)in rats based on serum metabonomics.Methods:A total of 28 SPF SD rats(half male and half female)were randomly divided into the blank group(11 rats)and the modelling group(17 rats).The model group was injected with type Ⅱ collagenase into the knee joint cavity on the 1st and4th day to establish the rat knee osteoarthritis models,while the blank group was injected with normal saline in the same way.The 16 rats with successful modeling were randomly divided into the model group and the group of Dampness-Resolving and Pain-Relieving Decoction(4.68 mg·kg-1),with 8 rats in each group.The rats in the blank group and the model group were given e-qual dose of normal saline by gavage,once a day,for 4 weeks.After the last gavage,the serum of rats in each group was collected,and the levels of interleukin-1β(IL-1β)and interleukin-18(IL-18)were detected by ELISA.The serum samples were detected by high-resolution time-of-flight mass spectrometry,and the clustering of each group of samples was judged by principal component analysis and partial least squares-discriminant analysis,so as to screen the differential metabolites and important KEGG pathways in serum.Results:Compared with the blank group,the serum 1L-1β level of the model group was significantly increased(P＜0.01).Compared with the model group,the serum IL-1β level of the group of Dampness-Resolving and Pain-Relieving Decoction was significantly decreased(P＜0.05).The serum metabolomics results showed that the metabolic profile of the model group and the blank group/the group of Damp-ness-Resolving and Pain-Relieving Decoction was significantly different.A total of 28 differential metabolites were identified,including corticosterone,ursodeoxycholic acid,p-cresol sulfate,etc.;6 important metabolic pathways were explored,such as steroidogenesis,gluta-mate metabolism,alanine metabolism,etc.Conclusion:Dampness-Resolving and Pain-Relieving Decoction may play a role in the treat-ment of KO A by regulating a variety of serum differential metabolites and metabolic pathways.
knee osteoarthritis(KOA)Dampness-Resolving and Pain-Relieving Decoctionmetabolomicsdifferential metabolitesste-roidogenesiscorticosteronerats
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