Study on the Mechanism of Method of Replenishing Qi and Warming Yang and Protecting Defense Qi Intervening the Level of Defensin in Asthmatic Rats Based on JAK/STAT Pathway
Objective:To study on the mechanism of method of replenishing qi and warming yang and protecting defense qi intervening the level of defensin in asthmatic rats based on Janus kinase(JAK)/signal transducer and activator of transcription(STAT)pathway.Methods:A total of 50 SPF male SD rats were randomly divided into the normal group(group A),the model group(group B),low-dose group of Decoction for Replenishing Qi and Warming Yang and Protecting Defense Qi(group C,5 g·kg-1),the medium-dose group of Decoction for Replenishing Qi and Warming Yang and Protecting Defense Qi,(group D,10 g·kg-1)and the high-dose group of De-coction for Replenishing Qi and Warming Yang and Protecting Defense Qi,(group E,20 g·kg-1),with 10 rats in each group.Except the group A,the other groups were given by intraperitoneal injection of Antigen Solution(ovalbumin+aluminum hydroxide)combined with atomization(Ovalbumin)excitation to establish the model of asthmatic rats.On the 8th day of modeling,the group C-E began to gavage the corresponding dose of Decoction for Replenishing Qi and Warming Yang and Protecting Defense Qi,while the group A and group B were gavaged with the same amount of saline,twice a day,for 4 weeks.The expression level of serum β-defensins was detected by RT-PCR;the levels of serum interferon gamma(IFN-γ)and interleukin-4(IL-4)were detected by ELISA;the expression levels of JAK mRNA and STAT6 mRNA in lung tissue were detected by RT-PCR;and the level of immune cells in bronchoalveolar lavage fluid was detected by Wright's staining method.Results:(1)Compared with the group A,the serum β-defensins expression level of rats in group B-E was significantly increased.Compared with the group B,the serum β-defensins expression level of rats in group C-E was sig-nificantly decreased(P＜0.05);there was no significant difference in the expression level of β-defensins in group C-E.(2)Compared with the group A,the serum IFN-γ level of rats in group B-E was significantly decreased.Compared with the group B,the serum IFN-γlevel of rats in group C-E was significantly increased.Compared with the group A,the serum IL-4 level of rats in group B-E was signifi-cantly increased.Compared with the group B,the serum IL-4 level of rats in group C-E was significantly decreased(P＜0.05);there were no significant differences in the serum IFN-y and IL-4 levels of rats in group C-E.(3)Compared with the group A,the JAK mRNA and STAT6 mRNA expression levels of lung tissue of rats in group B-E were significantly increased.Compared with the group B,the JAK mRNA and STAT6 mRNA expression levels of lung tissue of rats in group C-E were significantly decreased(P＜0.05);there were no sig-nificant differences in the JAK mRNA and STAT6 mRNA expression levels of lung tissue of rats in group C-E.(4)Compared with the group A,the levels of neutrophils,macrophages,eosinophils and lymphocytes in BALF of rats in group B-E were significantly increased.Compared with the group B,the levels of neutrophils,eosinophils,macrophages and lymphocytes in group C-E were significantly decreased(P＜0.05);there were no significant differences in the levels of neutrophils,eosinophils,macrophages and lymphocytes in group C-E.Conclusion:Method of replenishing qi and warming yang and protecting defense qi can reduce the inflammatory response and increase the expression level of β-defensins in asthmatic rats,and its mechanism may be related to the regulation of JAK/STAT signaling pathway.
bronchial asthmamethod of replenishing qi and warming yang and protecting defense qiJAK/STATdefensinIFN-γIL-4rats
国家科技期刊平台
NSTL
万方数据
