The Influence of Granules for Invigorating Circulation and Reducing Blood Lipid on Hyperlipidemia Model Rats Based on TLR4/MYD88/NF-κB Pathway
Objective:To study the influence of Granules for Invigorating Circulation and Reducing Blood Lipid(GICRBL)on hyperlipi-demia model rats based on Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MYD88)/nuclear transcription factor-κB(NF-κB)pathway.Methods:A total of 3 from 15 SD rats were randomly selected as the blank group,and the other rats were given high-fat diet for 2 weeks to establish the hyperlipidemia model.The successfully established model rats were randomly divided into the model group,the low-dose GICRBL group(1.56 mg·kg-1),the medium-dose GICRBL group(3.125 mg·kg-1),and the high-dose GI-CRBL group(6.25 mg·kg-1),with3 rats in each group.The rats in the corresponding groups were giventhe corresponding doses of drugs for 8 weeks,once a day,while the blank group and the model group were given normal saline by gavage.The serum lipid levels of rats were detected by automatic biochemical instrument,including total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C).The serum inflammatory factors were detected by ELISA,in-cluding tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1),and interferon-γ(IFN-γ).The protein and gene expression levels of TLR4,MYD88,and NF-κB in carotid artery tissue were detected by Western Blot and qPCR.Results:Com-pared with the blank group,the serum TC,TG and LDL-C levels of the model group were increased(P<0.01).Compared with the model group,the serum TC,TG and LDL-C levels of the low-dose GICRBL group,the medium-dose GICRBL group,and the high-dose GICRBL group were decreased(P<0.05).There was no significant difference in the serum HDL-C level among the groups(P>0.05).Compared with the blank group,the serum MCP-1,TNF-α,and IFN-γ contents of rats in the model group were significantly in-creased(P<0.01).Compared with the model group,the contents of MCP-1,TNF-α and IFN-γ in the serum of rats in the medium-dose and high-dose GICRBL groups were decreased(P<0.05),and the contents of TNF-α and IFN-γ in the serum of rats in the low-dose GI-CRBL group were decreased(P<0.05).Compared with the blank group,the expression levels of TLR4 mRNA,MYD88 mRNA,and NF-κB mRNA in the carotid artery tissue of rats in the model group were significantly increased(P<0.01).Compared with the model group,the expression levels of TLR4 mRNA,MYD88 mRNA and NF-κB mRNA in the carotid artery tissue of rats in the low-dose,medium-dose and high-dose groups of Jiangzhitongmaiyin were decreased(P<0.05).Compared with the blank group,the protein expression levels of TLR4,MYD88 and NF-κB in the carotid artery tissue of rats in the model group were significantly increased(P<0.01).Compared with the model group,the protein expression levels of TLR4,MYD88 and NF-κB in the carotid artery tissue of rats in the medium-dose and high-dose groups of GICRBL were significantly decreased(P<0.05).Conclusion:GICRBL can reduce the blood lipid level in hyperlipi-demic rats,and its mechanism may be related to inhibiting TLR4/MYD88/NF-κB pathway,thereby reducing inflammatory response.
hyperlipidemiagranules for invigorating circulation and reducing blood lipid(GICRBL)blood lipidTLR4/MYD88/NF-κB pathwayinflammationrats
万方数据
维普
