Protective Effect of Supplemented Trichosanthes and Chinese Chive Decoction on Acute Myocardial Injury in Rats
Objective:To observe the effects of Supplemented Trichosanthes and Chinese Chive Decoction on cardiac function indexes and myocardial histopathological morphology in rats with myocardial ischemia reperfusion injury(MIRI).Methods:Sixty-four Wistar rats were randomly divided into the sham operation group with 11 rats,the model group with 11 rats,the low-dose group of Trichosanthes and Chinese Chive Decoction(1.134 g·kg-1)with 11 rats,the medium-dose group of Trichosanthes and Chinese Chive Decoction(2.268 g·kg-1)with 11 rats,the high-dose group of Trichosanthes and Chinese Chive Decoction(4.536 g·kg-1)with10 rats and the group of compound Danshen dripping pill(86 mg·kg-1)with 10 rats.Except the sham operation group,the MIRI model was es-tablished by ligating the coronary artery in the other rats.After modeling,the rats in each drug group were given corresponding drugs by gavage,while the sham operation group and the model group were given normal saline by gavage,once a day,for 7 days.The contents of cardiac troponin T(CTNT),endothelin-1(ET-1)and thromboxane B2(TXB2)in serum were detected by ELISA method.The left ventricular internal dimension diameter(LVIDd),left ventricular ejection fraction(EF),left ventricular internal systolic diameter(LVIDs),cardiac output(CO),left ventricular internal diastolic volume(LVEDV),stroke volume(SV),left ventricular internal sys-tolic volume(LVESV)were measured by echocardiography.The pathological morphology of myocardial tissue was observed by HE stai-ning.Results:Compared with the sham operation group,the serum contents of CTNT,ET-1 and TXB2 in the model group were increased(P<0.05).Compared with the model group,the serum contents of CTNT,ET-1 and TXB2 in the medium-dose group of Trichosanthes and Chinese Chive Decoction and the group of compound Danshen dripping pill were decreased(P<0.05).Compared with the sham operation group,the LVIDd and LVEDV in the model group were increased(P<0.01),while the LVIDs,LVESV,SV,CO,and EF were decreased(P<0.05).compared with the model group,the LVIDd and LVEDV in the medium-dose group of Trichosanthes and Chinese Chive Decoction and the group of compound Danshen dripping pill were decreased(P<0.05),while the LVIDs,LVESV,SV,CO,and EF were increased(P<0.05).The HE staining results showed that the control group rats had well-arranged myocardial cells without inflammatory cell infiltration;the model group rats had a large number of irregularly arranged myocardial fibers,interstitial ede-ma and necrosis;the high-dose group of Trichosanthes and Chinese Chive Decoction had mildly swollen myocardial cells with more regu-lar myofibrils arrangement;the rat heart tissue in the group of compound Danshen dripping pill showed necrosis of myocardial cells un-der the pericardium,infiltration of neutrophils,and edema and congestion of the interstitial tissue.Conclusion:Supplemented Trichosan-thes and Chinese Chive Decoction has a significant protective effect on MIRI in rats.
Trichosanthes and Chinese Chive Decoctionmyocardial ischemia-reperfusion injury(MIRI)CTNTET-1TXB2ratsSyn-opsis of the Golden ChamberZhang Zhongjing
国家科技期刊平台
NSTL
万方数据
维普
