The Influence of Chaihuang Pancreatitis-Clearing Blood-Activating Granules on Microcirculation Disorders in Rat Models of Severe Acute Pancreatitis
Objective:To observe the influence of Chahuang Pancreatitis-Clearing Blood-Activating Granules on microcirculation disorders in rat models of severe acute pancreatitis(SAP).Methods:A total of 48 SD rats were randomly divided into the sham surgery group,the model group,and the group of Chaihuang Pancreatitis-Clearing Blood-Activating Granules,with 16 rats in each group.Except the sham surgery group,the remaining rats were injected with 3.5％sodium taurocholate in the pancreatic-biliary duct to replicate the SAP mod-els.After surgery,the group of Chaihuang Pancreatitis-Clearing Blood-Activating Granules was given the corresponding medicine by ga-vage(2 g·kg-1),while the rats in the sham surgery group and the model group were given the same dose of saline by gavage.Blood and pancreatic tissue were collected from the rats at the 12th hour and the 24th hour after the model was established.The level of serum amylase was detected by automatic biochemical analyzer;the pathological morphology of the pancreas was observed by HE staining,and the expression level of VEGF gene and protein in the pancreas was detected by RT-PCR and Western blot.Results:(1)After modeling,the serum amylase levels of the model group rats increased at the 12th hour and the 24th hour respectively compared with the sham sur-gery group(P＜0.05).At the 24th hour after modeling,compared with the model group,the serum amylase levels of the group of Chaihuang Pancreatitis-Clearing Blood-Activating Granules decreased(P＜0.05).(2)In the sham surgery group,the glandular struc-ture of the pancreas in rats was intact and no glandular necrosis was observed.In the model group,there was obvious glandular swelling,vascular rupture,and infiltration of inflammatory cells in the pancreas of rats.In the group of Chaihuang Pancreatitis-Clearing Blood-Ac-tivating Granules,there was glandular swelling,mild edema and infiltration of inflammatory cells in the pancreas of rats.(3)At the 12th hour and the 24th hour respectively after modeling,the pathological score of the pancreas in the model group was higher than that of the sham surgery group(P＜0.05);compared with the model group,the pathological score of the pancreas in the group of Chaihuang Pan-creatitis-Clearing Blood-Activating Granules was lower(P＜0.05).(4)At the 12th hour and the 24th hour respectively after model-ing,the VEGF gene and protein expression levels in the model group were higher than those in the sham surgery group(P＜0.05);compared with the model group,the VEGF gene and protein expression levels in the group of Chaihuang Pancreatitis-Clearing Blood-Ac-tivating Granules were lower(P＜0.05).Conclusion:Chaihuang Pancreatitis-Clearing Blood-Activating Granules can alleviate pancre-atic injury and microcirculatory obstruction in SAP rats,and its mechanism may be related to downregulating the expression level of VEGF in pancreatic tissue.
severe acute pancreatitis(SAP)Chaihuang Pancreatitis-Clearing Blood-Activating Granulesmicrocirculation disordersvascular endothelial growth factorrats
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