Study on the Mechanism of Liver-Soothing and Depression-Relieving Decoction in Improving Depressive Behavior in Ovariectomized Rats
Objective:To explore the intervention effects and mechanisms of Liver-Soothing and Depression-Relieving Decoction on de-pressive-like behaviors in(OVX)rat models with ovariectomy(OVX).Methods:A total of 60 SPF-grade Sprague-Dawley rats were randomly divided into the sham operation group,the model group,the fluoxetine group(10 mg·kg-1),the low dose(22.8 g·kg-1)and high-dose(45.6 g·kg-1)Liver-Soothing and Depression-Relieving Decoction groups,with 10 rats in each group.Except the sham operation group,the other rats underwent bilateral ovariectomy to establish the OVX model.After successful model establishment,the medication groups were administered the corresponding drugs for 6 weeks.Depressive-like behaviors were evaluated using the open field test and sucrose preference test.ELISA was used to detect levels of 5-hydroxytryptamin(5-HT),dopamine(DA),and norepinephrine(NE)in the brain tissue,as well as cortisol,adrenocorticotropic hormone(ACTH),and corticotropin-releasing hormone(CRH)levels in the serum.HE staining and Nissl staining were employed to observe the morphology of cells and Nissl bodies in the hippocampus.Western Blot analysis was conducted to detect the expression levels of brain-derived neurotrophic factor(BDNF)protein in the hippo-campus of each group.Results:Compared with the sham operation group,the model group showed significantly decreased sucrose prefer-ence,open field test activity,brain neurotransmitter levels,and BDNF expression(P<0.05),along with significantly increased serum HPA axis hormone levels(P<0.05).Compared with the model group,all medication groups showed significantly increased sucrose consumption,open field test activity,brain neurotransmitter levels,and BDNF expression(P<0.05),while serum HPA axis hormone levels were significantly decreased(P<0.05).Pathological observations indicated that the morphology and number of neurons,as well as the number of Nissl bodies in the hippocampus,were notably improved in all medication groups.Conclusion:Liver-Soothing and De-pression-Relieving Decoction can improve the depressive state in OVX rats,and its mechanism may be closely related to the promotion of BDNF expression in hippocampal neurons.
Liver-Soothing and Depression-Relieving Decoctiondepressionovariectomybrain-derived neurothrophic factor(BDNF)rats
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