The Effects of Tibetan Medicine ByudMar 25 on Motor Function and Oxidative Stress Levels in Model Rats with Parkinson's Disease
Objective:To explore the effect of Tibetan medicine ByudMar 25(BM25)on motor function and oxidative stress levels in rat models with Parkinson's disease(PD).Methods:A total of 24 SD rats were randomly divided into the ByudMar 25 group(180 mg·kg-1),the model group,and the normal group,with 8 rats in each group.Except for the normal group,PD models were es-tablished in the other rats via unilateral intrastriatal injection of 6-hydroxydopamine(6-OHDA).After successful modeling,rats in each group were administered their respective treatments by gavage once daily for 4 weeks,while the model and normal groups received an e-quivalent volume of normal saline.The body weight of the rats was measured on the 1st day,7th day,14th day,21st day,and 28th of treatment.After 4 weeks,behavioral changes were observed using the rotation test and open-field test.ELISA was used to measure the levels of malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD)in the striatum of the rats.Results:There were no significant differences in body weight among the groups(P>0.05).Compared with the normal group,the model group showed a significant increase in rotation frequency(P<0.001),a significant decrease in average speed in the open-field test(P<0.05),a significant reduction in movement distance(P<0.01),and a significant extension of resting time(P<0.05).Additionally,the MDA levels in the striatum significantly increased(P<0.05),while the levels of SOD and GSH significantly decreased(P<0.05).Com-pared with the model group,the ByudMar 25 group showed a significant decrease in rotation frequency(P<0.05),a significant in-crease in average speed(P<0.05),a significant extension of movement distance(P<0.05),and a significant reduction in resting time(P<0.05).MDA levels in the striatum significantly decreased(P<0.05),while GSH and SOD levels significantly increased(P<0.05).Conclusion:ByudMar 25 can significantly improve the motor abilities of PD rats and regulate oxidative stress in the brain.
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