Astragaloside Ⅳ and Panax notoginseng saponins combined with Bingpian(Borneolum Syntheticum)in improving renal injury after cerebral ischemia-reperfusion in rats
Objective To observe the effects of astragaloside Ⅳ(AST Ⅳ)and Panax notoginseng saponins(PNS)combined with Bingpian(Borneolum Syntheticum)in improving renal injury after cerebral ischemia-reperfusion in rats.Methods A focal cerebral ischemia model was established by middle cerebral artery occlusion method.The rats were randomized into sham-operated group,model group,Bingpian(Borneolum Syntheticum)group,ASTⅣcombined with PNS(AP)group,and ASTⅣand PNS combined with Bingpian(Borneolum Syntheticum)(APB)group.After two hours of ischemia,reperfusion was performed for 48 hours.Neurological function was graded.HE staining and Nissl staining were used to determine brain tissue function and nerve cell damage.In addition,HE staining was used to measure renal injury.Renal function was evaluated by renal mass index and the content of creatinine and urea nitrogen in serum.Moreover,the content of IL-1β and IL-10 was checked by ELISA,NF-κB protein expression in the renal tissue was examined by immunohistochemistry method,and TLR4 and MyD88 protein expressions in the renal tissue were determined by Western blot.Results Compared with sham-operated group,the neurological function score and nerve cell injury rate in model group significantly increased(P<0.01),and the number of Nissl bodies significantly decreased(P<0.01).Additionally,renal tubular edema and nuclear pyknosis were observed,and renal mass index decreased(P<0.01).The content of serum creatinine and urea nitrogen increased(P<0.01).It is suggested that cerebral ischemia-reperfusion can induce secondary renal injury.Compared with model group,all drug groups showed reduced neurological function scores(P<0.01),decreased nerve cell injury rate(P<0.01 or P<0.05),increased number of Nissl bodies(P<0.01),improved renal structural damage,elevated renal mass index(P<0.01 or P<0.05),and lower content of serum creatinine and urea nitrogen(P<0.01 or P<0.05)to varying degrees.APB group had the best effects in these above changes.Compared with sham-operated group,the content of IL-1β in serum and the protein expression levels of NF-κB,TLR4,and MyD88 in the renal tissue of model group increased(P<0.01).Compared with model group,all drug groups showed a decrease in the serum IL-1β content(P<0.01 or P<0.05)and protein expression levels of NF-κB,TLR4,and MyD88 in the renal tissue(P<0.01 or P<0.05),and an increase in the serum IL-10 content(P<0.01 or P<0.05).Conclusion Cerebral ischemia-reperfusion can induce secondary renal injury,and APB can not only alleviate brain tissue damage after cerebral ischemia-reperfusion,but also reduce secondary renal injury after it.Its effects are related to the regulation of the TLR4/MyD88/NF-κB signaling pathways and the inhibition of renal inflammation.
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万方数据
维普
