Expression and significance of CFH protein in familial premature coronary heart disease with blood stasis pattern
Objective To screen the specific proteins of familial premature coronary heart disease(PCHD)with blood stasis pattern,and to provide potential biomarkers for early clinical diagnosis and prevention of the disease in the future.Methods The plasma protein expressions of familial PCHD patients with blood stasis pattern,familial PCHD patients without blood stasis pattern,and healthy control groups were analyzed by isobaric tags for relative and absolute quantification(iTRAQ).After data analysis,the differential protein expressions among the groups were obtained to preliminarily get the predicted protein of the disease by the differential protein screening criteria.The predicted protein was verified by Western blot.Results A total of 75 differential proteins were obtained by iTRAQ,of which 32 differential proteins were obtained between the familial PCHD with blood stasis pattern group and the healthy control group,in cluding 22 up-regulated proteins and 10 down-regulated proteins,involving 429 biological processes,which were most closely related to keratinization,skin development,and protein activation cascade reaction;they were enriched in KEGG pathways such as Staphylococcus aureus infection,complement and coagulation cascades,and platelet activation.Compared with the healthy control group,the difference of the expression level of complement factor H(CFH)protein in the familial PCHD with blood stasis pattern group had statistical significance(P<0.01).Conclusion The CFH protein obtained by the differential protein screening criteria can be used as a potential biomarker for the early diagnosis of familial PCHD with blood stasis pattern.
premature coronary heart diseaseblood stasis patternisobaric tags for relative and absolute quantificationcomplement factor H
国家科技期刊平台
NSTL
万方数据
维普
