Effects of Sanbu Decoction on cognitive function and lipid peroxidation damage in naturally aging mice
Objective To observe the effects of Sanbu Decoction(SBD)on the cognitive function of naturally aging mice,and to explore the involved potential pathological factors,as well as the action of SBD and its possible mechanism.Methods Eighteen healthy SPF wild-type C57BL/6 naturally aging mice,half female and half male,aged 18 months,were randomized into aging group(WT-Aged),donepezil group(WT-Donepezil),SBD group(WT-SBD),and another six mice aged three months were selected as the young control group(WT-Young).WT-Young group was not treated.WT-Aged group were treated with distilled water,and the rest groups with corresponding drugs,by gavage for 45 days.During the six days before the end of gavage,Morris water maze test was performed,and the escape latency,platform crossing times,and the swimming distance in the platform quadrant were recorded.HE staining was carried out to observe the morphologic changes of hippocampal neurons of the mice.Immunohistochemistry was applied to measuring the expression levels of Tau,p-Tau,glial fibrillary acidic protein(GFAP),and neuroepithelial stem cell protein(Nestin)in the hippocampal tissue of the mice.The detection kits were used to check the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA).Results Compared with WT-Young group,the escape latency of WT-Aged group was prolonged from the 2nd day(P<0.05),and the platform crossing times and the swimming distance in the platform quadrant were reduced(P<0.05);compared with WT-Aged group,the escape latency of WT-Donepezil group on the 3rd to 5th day and WT-SBD group on the 4th to 5th day was shortened(P<0.05),and platform crossing times and the swimming distance in the platform quadrant were increased(P<0.05).The hippocampal neurons in WT-Aged group showed disordered arrangement and large areas of necrosis,enhanced acidophilia in the cytoplasm,and pyknosis,dissolution,and disappearance of the nuclei.Compared with WT-Aged group,the hippocampal neurons in WT-Donepezil and WT-SBD groups showed reduced damage and compact arrangement,and the degenerated and pyknotic cells were significantly decreased,with a small minority showing karyopyknosis and cytoplasmic acidophilic change.Compared with WT-Young group,the expressions of Tau,p-Tau,and GFAP in WT-Aged group increased(P<0.05),the expression of Nestin and the activity of SOD and GSH-Px decreased(P<0.05),and the content of MDA was elevated(P<0.05);compared with WT-Aged group,the expressions of p-Tau and GFAP decreased in WT-Donepezil and WT-SBD groups(P<0.05),and the activity of SOD and GSH-Px in WT-SBD group increased(P<0.05)while the content of MDA was lowered(P<0.05).Conclusion SBD can reduce the expression of p-Tau in the hippocampus of naturally aging mice,weaken the activation level of astrocytes,and reduce lipid peroxidation,which can improve the learning and memory abilities of senile mice,alleviate their aging state,and delay the aging process,thus playing the roles of brain protection and anti-aging.
万方数据
维普
