Chemical components of coumarins in Yunxiang(Ruta graveolens L.)
Objective To study the chemical components of coumarins in Chinese medicine Yunxiang(Ruta graveolens L.)and explore its anti-inflammatory and anticoagulant activities.Methods Various chromatographic separation techniques,combined with nuclear magnetic resonance spectroscopy and mass spectrometry were used in this experiment to identify the structures of the isolated monomeric compounds.The CCK-8 method was used to screen out compounds with non-significant cytotoxicity for the measurement of nitric oxide(NO)released by lipopolysaccharide(LPS)-induced mouse macrophages(RAW 264.7),as well as for anti-inflammatory activity screening.The prothrombin time(PT)was performed to screen for anticoagulant activity.Results Ten coumarin compounds were identified from Yunxiang(Ruta graveolens L.)extract,namely,psoralen(1),xanthotoxin(2),chalepensin(3),rutamontine(4),3-(1',1',dimethyl allyl)-herniarin(5),bergapten(6),isoscopoletin(7),isofraxidin(8),demethylsuberosin(9),and chalepin(10).Evaluation of the anti-inflammatory and anticoagulant activities of these compounds showed that compounds 2,4,and 9 exhibited significant inhibitory effects on the production of NO induced by LPS at a concentration of 20 μmol/L,with inhibition rates of 54.3±1.7%,51.2±5.5%,and 52.3±1.7%,respectively.Besides,none of the ten compounds demonstrated anticoagulant activity at a concentration of 1.0 mmol/L.Conclusion Ten monomeric coumarin compounds were isolated from the extract of Yunxiang(Ruta graveolens L.),with compounds 3-5 and 7-10 being isolated from Yunxiang(Ruta graveolens L.)for the first time.This study enriches the research on the chemical composition and biological activity of Yunxiang(Ruta graveolens L.),providing a theoretical basis for the development and utilization of its anti-inflammatory activity.
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