Quality evaluation of Baizhu Houpo Mixture based on multi-index component quantification,chemometrics,and EW-TOPSIS analysis
Objective Ten components were selected as indicators to establish a high performance liquid chromatography(HPLC)quantitative analysis method for Baizhu Houpo Mixture,and to comprehensively evaluate its quality through chemometrics and entropy weight solution distance method(EW-TOPSIS).Methods The 75%methanol extract was used as the sample,and the Agilent TC-C18 chromatographic column was used with acetonitrile-0.1%phosphoric acid as the mobile phase for gradient elution.HPLC was used to simultaneously determine the content of ten compounds(atractylenolide Ⅲ,atracylenolide Ⅰ,cinnamyl alcohol,cinnamic acid,cinnamaldehyde,honokiol,magnolol,6-gingerol,8-gingerol,and 10-gingerol)in 12 batches of Baizhu Houpo Mixture,and the content data were analyzed by chemometrics and EW-TOPSIS method.Results The methodological verification showed that all the indexes met the requirements of Chinese Pharmacopoeia(ten components had good linear relationships in their respective mass concentration ranges,with correlation coefficients＞0.999;the RSDs of precision,stability,repeatability and recovery rate were all<2.0%).Chemometrics analysis revealed that the first two principal components represented 92.24%of the information of the ten components,and the 12 batches of samples were clustered into three categories.Cinnamaldehyde,magnolol,honokiol,atractylenolide III,6-gingerol and atractylenolide I had a great influence on the quality of Baizhu Houpo Mixture.The results of the EW-TOPSIS method showed that the average Euclidean proximity of the optimal solution among the 12 batches of Baizhu Houpo Mixture ranged from 0.238 5-0.666 2,and the quality of each batch was quite different.Conclusion The established multi-index component quantification method is convenient to operate and accurate,and can be used for quality control of Baizhu Houpo Mixture.Moreover,chemometrics and EW-TOPSIS analysis can realize the comprehensive evaluation of the quality of Baizhu Houpo Mixture,providing a reference for the improvement of its quality standards.
万方数据
维普
