Effects of Xuebijing Injection on inflammatory response and cell apoptosis in rats with LPS-induced acute lung injury
Objective To observe the effects of Xuebijing Injection(XBJI)on inflammatory response and cell apoptosis in rats with lipopolysaccharide(LPS)-induced acute lung injury(ALI).Methods A total of 30 male SD rats were randomized into normal group(n=10)and modeling group(n=20)which was subdivided randomly into model group(n=10)and XBJI group(n=10)after modeling(injection of LPS into the tail vein).The degree of pulmonary edema was evaluated using the wet/dry weight ratio(W/D)of the lung tissue,the serum levels of inflammatory factors IL-6,IL-1β,IL-4,and IL-18 were determined by ELISA,the pathological damage of rat lung tissue was observed by HE staining,the condition of cell apoptosis was observed by TUNEL staining,and the mRNA content and protein levels of apoptotic factors Caspase-3 and Caspase-8 were measured by RT-PCR and Western blot.Results Compared with the normal group,the model group showed an increase in the rat lung tissue W/D(P<0.01),levels of IL-6,IL-1β,IL-4,and IL-18(P<0.001),cell apoptosis rate(P<0.001),as well as the mRNA and protein expressions of Caspase-3 and Caspase-8(P<0.01 or P<0.001);besides,the lung tissue was damaged with inflammatory cell infiltration.Compared with the model group,the XBJI group exhibited a decrease in the lung tissue W/D(P<0.05),levels of IL-6,IL-1β,IL-4,and IL-18(P<0.05 or P<0.01),cell apoptosis rate(P<0.001),as well as the mRNA and protein expressions of Caspase-3 and Caspase-8(P<0.01 or P<0.001);additionally,the damage to the lung tissue and inflammatory cell infiltration were significantly alleviated.Conclusion XBJI can relieve the lung tissue injury and inflammatory response in ALI rats,the mechanism of which may be related to inhibiting cell apoptosis.
万方数据
维普
