Effects of Yupingfeng Powder on expression levels of IL-4,IL-12,and TNF-γ as well as nasal mucosa pathological changes in guinea pig model of allergic rhinitis with lung qi deficiency pattern
Effects of Yupingfeng Powder on expression levels of IL-4,IL-12,and TNF-γ as well as nasal mucosa pathological changes in guinea pig model of allergic rhinitis with lung qi deficiency pattern
Objective To explore the action of mechanism of Yupingfeng Powder(YPFP)on allergic rhinitis(AR)with lung qi deficiency pattern in guinea pigs.Methods Forty healthy variegated British guinea pigs were randomized into blank group,model group,YPFP group,and loratadine group.Except for the blank group,the AR models with lung qi deficiency pattern were established in the other groups using ovalbumin(OVA)and smoke exposure.After successful modeling,the YPFP group and the loratadine group were treated with YPFP[4.52 g/(kg·d)]and loratadine tablets(0.903 mg/kg)by gavage,respectively,while the blank group and model group were given an equal volume of normal saline by gavage.After 15 days of treatment,the serum and nasal mucosa samples of guinea pigs were collected from each group.The morphology of nasal mucosa cells were observed using HE staining,and the expression levels of cytokines such as interleukin-4(IL-4),interleukin-12(IL-12),and interferon-γ(IFN-γ)in the serum of guinea pigs were measured by ELISA and immunohistochemical staining.Results HE staining showed that the model group exhibited significant morphological and structural disorders,while the morphological and structural abnormalities were relatively alleviated in the YPFP group and the loratadine group.ELISA results indicated that compared with the blank group,the expression levels of IL-4 and IL-12 in the serum of guinea pigs in the model group,YPFP group,and loratadine group increased(P<0.05),while the expression level of IFN-γ decreased(P<0.05).Compared with the model group,the serum levels of IL-4 and IL-12 in the YPFP group and loratadine group decreased(P<0.05),while the expression level of IFN-γ in the loratadine group increased(P<0.05).Immunohistochemical staining revealed that compared with the blank group,the expressions of IL-4 and IL-12 in the nasal mucosa of guinea pigs in the model group were significantly lower(P<0.05),while the expression of IFN-γ was significantly higher(P<0.01).Compared with the model group,the IL-4 expression decreased significantly in both the YPFP group and loratadine group(P<0.05),and the IL-12 expression in the loratadine group decreased significantly(P<0.05).Conclusion YPFP may treat allergic rhinitis with lung qi deficiency pattern by influencing the levels of IL-4,IL-12,and IFN-γ,and changing the morphology of nasal mucosa cells.
关键词
玉屏风散/变应性鼻炎/肺气虚型/鼻腔黏膜细胞形态/白细胞介素
Key words
Yupingfeng Powder/allergic rhinitis/lung qi deficiency pattern/morphology of nasal mucosa cells/interleukin
维普
万方数据