Effects of Xuesaitai on protein expressions of STIM1 and Orai1 in ischemic stroke rats
Objective To study the effects of Xuesaitai on expressions of stromal interaction molecule 1(STIM1)and calcium release-activated calcium channel protein 1(Orai1)in ischemic stroke(IS)rats.Methods A total of 60 SPF-grade male SD rats were randomized into sham-operated group(n=10)and model group(n=50).A middle cerebral artery occlusion(MCAO)model was prepared by thread embolism.A total of 41 successfully modeled rats were randomly subdivided into model group,low-,medium-,and high-dose(12.6 g/kg,25.2 g/kg,50.4 g/kg)Xuesaitai groups,and enteric-coated aspirin tablets group(18 mg/kg),with nine rats in low-dose Xuesaitai group and eight rats in each of the other groups.The sham-operated and model groups were given an equal volume of normal saline by gavage for continuous seven days.The modified Neurological Severity Score(mNSS)was used to assess the neurological damage in rats from each group,TTC staining was used to determine the infarct area of rat brain tissue,ELISA was used to measure the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in rat serum,RT-PCR was used to check the mRNA expressions of STIM1 and Orai1 in brain tissue,and immunoprecipitation method was used to determine the relative protein expression levels of STIM1 and Orai1 in the brain tissue.Results Compared with the sham-operated group,the model group showed increased mNSS scores(P<0.01),enlarged infarct area(P<0.01),elevated serum levels of IL-6 and TNF-α(P<0.01),and higher mRNA and relative protein expression levels of STIM1 and Orai1 in the brain tissue(P<0.01).Compared with the model group,the rats in each dose group of Xuesaitai as well as enteric-coated aspirin tablets group showed decreased mNSS scores(P<0.05),reduced serum levels of IL-6 and TNF-α(P<0.01),and decreased mRNA expression levels of STIM1 and Orai1 in the brain tissue(P<0.01).Additionally,the rats in the medium-and high-dose Xuesaitai groups,and enteric-coated aspirin tablets group exhibited decreased infarct area(P<0.01)and reduced relative protein expression levels of STIM1 and Orai1 in the brain tissue(P<0.05,P<0.01).The high-dose Xuesaitai group exhibited lower cerebral infarct rate(P<0.01),significantly reduced serum levels of IL-6 and TNF-α(P<0.01),and significantly decreased mRNA expression levels of STIM1 and Orai1 in the brain tissue(P<0.01).Compared with the low-and medium-dose Xuesaitai groups,the high-dose Xuesaitai group exhibited reduced mNSS scores(P<0.05,P<0.01),lower cerebral infarct rate(P<0.01),reduced serum levels of IL-6 and TNF-α(P<0.01),and decreased mRNA expression levels of STIM1 and Orai1 in the brain tissue(P<0.01).Conclusion Xuesaitai can inhibit the expression and binding of STIM1 and Orai1,alleviate the inflammatory response,relieve neurological deficits,and reduce neuronal damage in MCAO rats,thus exerting an anti-IS effect.
ischemic strokeXuesaitaistromal interaction molecule 1calcium release-activated calcium channel protein 1inflammation
万方数据
维普
