Effects of Guben Pingchuan Formula on MUC5AC level in the airway of chronic obstructive pulmonary disease model rats
Objective Using Guben Pingchuan Formula(GBPCF)to intervene the rat model established by cigarette smoke exposure combined with tracheal instillation of lipopolysaccharide(LPS)and porcine pancreatic elastase(PPE),taking mucin 5AC(MUC5AC)as the evaluation indicator,so as to measure its content,protein expression,and mRNA expression in the rat lung tissue and evaluate the airway mucus secretion intensity in each group.Methods After one week of adaptive feeding of 64 SPF-level SD rats,52 of them were randomly taken for modeling.After successful modeling,50 model rats were randomized into model group(distilled water),dexamethasone group(0.15 mg·kg-1),and GBPCF high-(28 g·kg-1),medium-(14 g·kg-1),and low-dose groups(7 g·kg-1),with ten rats in each group.Another ten rats were selected as control group(distilled water).After 15 days,the rats were euthanized and specimens were collected.HE staining was used to observe the pathological changes in the lung tissue of rats,ELISA to determine the level of MUC5AC in the lung tissue,RT-PCR to measure MUC5AC mRNA expression level,Western blot to examine MUC5AC protein expression,and immunofluorescence to check the distribution pattern of MUC5AC expression.Results The pulmonary tissue structure of the model group was notably damaged,with a large amount of inflammatory infiltration in the alveolar cavity and pulmonary interstitium.The degree of alveolar damage and tissue inflammatory infiltration was reduced in the dexamethasone group and GBPCF groups of various doses.Compared with the control group,the content of MUC5AC in all other groups increased(P<0.05).Compared with the model group,the content and mRNA expression of MUC5AC in the dexamethasone group and GBPCF groups of various doses decreased(P<0.05),and the MUC5AC protein expression in the dexamethasone group,the high-and medium-dose groups of GBPCF decreased(P<0.05).Immunofluorescence of the lung tissue revealed strong MUC5AC expression in the model group,weaker expression in the dexamethasone group,and successively decreased expressions in the low-,medium-,and high-dose groups of GBPCF.Conclusion GBPCF can alleviate the pathological damage of lung tissue in model rats of chronic obstructive pulmonary disease(COPD),effectively reduce MUC5AC content,downregulate MUC5AC mRNA expression,decrease MUC5AC protein expression,and improve the high secretion state of airway mucus in rats.
万方数据
维普
