Protective effects of Guilu Erxian Glue on rats with oligoasthenospermia based on HIF-1α/EGFR/MAPK1 signaling pathway
Objective To explore the protective effects of Guilu Erxian Glue(GLEXG)on rats with oligoasthenospermia(OAS)by regulating the hypoxia inducible factor-1α/epidermal growth factor receptor/mitogen-activated protein kinase 1(HIF-1α/EGFR/MAPK1)signaling pathway.Methods Forty-eight male SD rats were randomized into normal,model control,low-dose GLEXG(0.65 g/kg),medium-dose GLEXG(1.25 g/kg),high-dose GLEXG(2.5 g/kg),and L-carnitine groups,with eight rats in each group.All groups except the normal group were administered adenine by gavage to create the model After successful modeling,the rats were intervened by intragastric administration for continuous four weeks,during which the general condition of the rats was recorded.After four weeks of administration,the rats were processed for sample collection.Blood was collected from the abdominal aorta.Serum testosterone(T),estradiol(E2),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and prolactin(PRL)levels were measured by ELISA.Renal tissues were separated to calculate the renal index.The right epididymis was separated,and the sperm concentration and motility of each group were checked by automatic sperm analyzer.The pathological changes of testicular tissue were observed by HE staining.Reactive oxygen species(ROS),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)levels in rat testicular tissue were examined by respective assay kits.Protein expressions of HIF-1α,EGFR,and MAPK1 in rat testicular tissue were checked by immunohistochemistry.Results Compared with the model control group,different doses of GLEXG significantly increased the body mass of rats(P<0.01),decreased their renal index of rats(P<0.01),improved sperm concentration and motility(P<0.01),thereby enhancing the sperm quality of rats and effectively reducing the degree of testicular tissue damage in the model rats.Compared with the low-dose GLEXG group,the high-dose group showed significant increase in body mass,sperm density,and sperm motility(P<0.05).ELISA results showed that compared with model control group,different doses of GLEXG significantly increased T and E2 levels(P<0.05),and significantly decreased FSH,LH,and PRL levels(P<0.05).The results of ROS,SOD,and GSH-Px indicated that compared with the model control group,ROS levels in low-,medium-,and high-dose GLXEG groups were significantly lower(P<0.05),while SOD and GSH-Px activities in medium-and high-dose GLXEG,and L-carnitine groups were significantly higher(P<0.05).Immunohistochemical results showed that compared with the model control group,the levels of HIF-1α,EGFR,and MAPK1 in rat testicular tissue of medium-,high-dose GLXEG and L-carnitine groups were significantly lower(P<0.05).Conclusion GLEXG may improve OAS by regulating oxidative stress through HIF-1α/EGFR/MAPK1 signaling pathway.
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维普
