Effects of Jingling Wuzao Solid Beverage on pulmonary inflammatory factors in mice with COPD
Objective To observe the effects of Jingling Wuzao Solid Beverage(JLWZSB)on the inflammatory response in the lung tissue of mouse models of chronic obstructive pulmonary disease(COPD),to explore its possible mechanism of action,to provide an experimental evidence for the clinical application of this formula,and to offer some insights for the prevention and treatment of COPD with Chinese medicinal diet therapy.Methods Twenty-four male C57BL/6 mice were randomized into normal,model,JLWZSB(2.5 g/kg),and dexamethasone(1.0 mg/kg)groups,with six mice in each group.All groups except the normal group were exposed to nasal drops of lipopolysaccharide combined with cigarette smoke to establish the COPD mouse models.Samples were collected after 30 days of intervention.HE staining was used to observe the pathological morphological changes in the lung tissue and adipose tissue iWAT and BAT of each group;ELISA was employed to measure the levels of tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in mouse serum and bronchoalveolar lavage fluid(BALF);qRT-PCR was conducted to check the relative mRNA expression levels of TNF-α,ICAM-1,SELE,SP-A,and SP-D in lung tissue of mice.Results Compared with the normal group,the model group mice showed a significant infiltration of inflammatory cells,destruction and fusion of alveolar walls resulting in pulmonary bullae,the diameter of adipocytes was larger in the model group of mice,and a significant increase in the concentrations of TNF-α and IL-6 in serum and BALF(P<0.01).The mRNA expression levels of TNF-α,ICAM-1,and SELE were significantly elevated(P<0.01).Compared with the model group,the JLWZSB and the dexamethasone groups showed reduced lung tissue damage,iWAT cells become smaller and tighter with a tendency to brown out,BAT cells become more numerous and tightly arranged in distribution,and decreased levels of TNF-α and IL-6 in serum(P<0.05),as well as reduced mRNA levels of TNF-α,ICAM-1,SELE,and SP-A(P<0.05).Conclusion JLWZSB can improve the lung tissue morphology,inhibit inflammatory responses,and promote lung tissue repair in COPD mouse models.
Jingling Wuzao Solid Beveragechronic obstructive pulmonary diseaseairway inflammationinflammatory factormouse modelhomology of medicine and food
万方数据
维普
