Preparation of betulin nanoemulsion and its distribution in S180 tumor-bearing mice
Objective To prepare betulin nanoemulsion and study its distribution in S180 tumor-bearing mice.Methods Betulin nanoemulsion was prepared by phase transition method.After intraperitoneal injection of betulin nanoemulsion and betulin injection,HPLC method was used to determine the drug concentration in various tissues of mice and calculate the pharmacokinetic parameters.The tissue distribution of the two preparations in mice was analyzed and compared.Results The Cmax and AUC of betulin nanoemulsion in each tissue were as follows:tumor tissue>liver>kidney>spleen>lung>heart,all higher than those in betulin injection group(P<0.05);the peak concentration ratios(Ce)in tumor tissue and liver were 1.53 and 1.54,respectively;the tumor targeting efficiency(Te)value was the highest.Conclusion Compared with betulin injection,betulin nanoemulsion significantly increased distribution in tumor tissues and liver(P<0.01),and betulin nanoemulsion exhibited obvious targeting towards tumor tissues.
万方数据
维普
