Pathological differences and Chinese medicine pattern evaluation of three rat models of heart failure
Objective To compare rat models of heart failure induced by doxorubicin(DOX),hypertension,and isoproterenol(ISO)in terms of modelling process and pathological features and to evaluate their Chinese medicine patterns to assist researchers in selecting appropriate models.Methods Thirty male Dahl salt-sensitive rats were used as the hypertensive heart failure model group(8%NaCl high-salt chow fed continuously for 20 weeks);sixty male SD rats were divided randomly and equally into the DOX heart failure model(DOX 1.5 mg/kg,intraperitoneally injected twice weekly for 7 weeks)and the ISO heart failure model groups[ISO 5 mg/(kg·d),intraperitoneally injected for 7 consecutive days and then continued feeding for another 3 weeks];and another 10 male SD rats were used as blank control group.HE staining was used to dye the hearts,kidneys,and livers of rats and Masson staining was used to dye cardiomyocytes;serum levels of N-terminal pro-brain natriuretic peptide(NT-proBNP)were determined by ELISA;and the cardiac function of rats was evaluated by echocardiography.Combined with the natural state and general signs,the Chinese medicine patterns of three heart failure rat models were also explored.Results Compared with the blank control group,the rats in the DOX heart failure model group had reduced activity,decreased body temperature,and obvious edema;the rats in the hypertensive heart failure model group showed lassitude,reduced urine output,and edema;and rats in the ISO heart failure model group showed reduced activity,decreased body temperature,and loose stool.The DOX and hypertensive heart failure model groups showed relatively serious pathological damage in the kidneys,while the ISO heart failure model group only had heart damage.Serum levels of NT-proBNP of the three heart failure model groups all increased(P<0.05),while left ventricular ejection fractions(LVEF)and left ventricular fraction shortening(LVFS)both decreased(P<0.05).The Chinese medicine pattern of the DOX heart failure model and ISO heart failure model groups was yang deficiency,while that of the hypertensive heart failure model group was dual deficiency of qi and yin.Conclusion According to the macroscopic manifestations and microscopic indexes of the rats,the three models have great differences in pathology,and thus careful selection is necessary.DOX and ISO heart failure models may be yang deficiency pattern,while hypertensive heart failure models may be dual deficiency of qi and yin pattern.
heart failurerat modeletiological modellingpathological differenceChinese medicine patternmodel eval-uation
万方数据
维普
