Effects of Hedyotis diffusa polysaccharide on proliferation,migration,and invasion of breast cancer cells by regulating CXCL12/CXCR4 axis
Objective To study the effects of Hedyotis diffusa Willd.Polysaccharide(HDP)on proliferation,invasion,and migration of breast cancer cells MDA-MB-231 and the regulatory mechanism of HDP on CXCL12/CXCR4 axis.Methods Breast cancer cells MDA-MB-231 were assigned into control group,CXCL12/CXCR4 inhibitor group(AMD 3100 group),and low-,medium-,and high-dose HDP groups(HDP-L group,HDP-M group and HDP-H group),and HDP-H+CXCL12 group(HDP-H+CXCL12 group).CCK-8 was used to determine cell proliferation.Scratch assay and Transwell assay were applied to evaluate the cell migration and invasion ability.Western blot was employed to examine the protein expressions of E-cadherin,Vimentin,CXCL12,and CXCR4.MDA-MB-231 transplanted tumor model of BALB/c-nu female nude mice were established and the tumor growth of mice in each group was observed.HE staining was used to observe the morphological changes of transplanted tumor cells in nude mice.Results Compared with the control group,the cell proliferation activity,migration,and invasion number,as well as the expression levels of Vimentin,CXCL12,and CXCR4 proteins in AMD 3100 group and HDP groups of various doses decreased,while the expression level of E-cadherin protein distinctly increased(P<0.05,P<0.01).Compared with the HDP-H group,the cell proliferation,migration,and invasion number,as well as the expression levels of Vimentin,CXCL12,and CXCR4 proteins in HDP-H+CXCL12 group were enhanced(P<0.01),while the expression level of E-cadherin protein decreased(P<0.01).Additionally,in xenograft tumor experiments,compared with the control group,the tumor weight of AMD 3100 group and HDP groups of various doses were significantly lower(P<0.05,P<0.01);the tumor weight of HDP-H+CXCL12 group was significantly higher(P<0.01).HE staining showed the increased necrosis and apoptosis of tumor tissue cells in each intervention group of AMD 3100 and HDP.Conclusion HDP may inhibit the proliferation,migration,and invasion of MDA-MB-231 cells by regulating CXCL12/CXCR4 axis.
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