Mechanism of action of Ziyin Mingmu Formula medicated serum on tunicamycin-induced ARPE-19 cells based on PERK/ATF4/CHOP signaling pathway
Objective To investigate the effects of Ziyin Mingmu Formula(ZYMMF)medicated serum on tunicamycin(TM)-induced ARPE-19 cells and its potential mechanism.Methods The endoplasmic reticulum stress injury model was constructed and ARPE-19 cells were divided into blank control,model,blank control serum,ZYMMF medicated serum,and taurodeoxycholic acid groups.Cell morphology was observed,cell survival rate was determined by CCK-8,cell apoptosis was tested by TUNEL,and protein expressions of PERK kinase(PERK),activating transcription factor 4(ATF4),and transcription factor CHOP(CHOP)were measured by Western blot.Results ARPE-19 cells were treated with 50 μmol/L tunicamycin for modeling.Compared with the model group,the number of ARPE-19 cells in ZYMMF medicated serum and taurodeoxycholic acid groups increased with more uniform growth and reduced floating dead ARPE-19 cells and fragments.Compared with blank control group,the cell survival rate in model group and blank control serum group decreased(P<0.01),the apoptosis rate increased significantly(P<0.01),and the protein expressions of PERK,ATF4,and CHOP were significantly up-regulated(P<0.01).Compared with the model group,the survival rate of ZYMMF medicated serum group increased(P<0.01),the apoptosis rate decreased significantly(P<0.01),and the protein expressions of PERK,ATF4,and CHOP were significantly down-regulated(P<0.01).Conclusion ZYMMF medicated serum can reduce the apoptosis of endoplasmic reticulum stress injury model of ARPE-19 cells,and its molecular mechanism is related to the regulation of PERK/ATF4/CHOP signaling pathway.
万方数据
维普
