Experimental study on construction of rat model of lower back pain due to cold dampness stagnation and intervention effects of Duhuo Jisheng Decoction
Objective To construct the rat model of lower back pain due to cold dampness stagnation,and to evaluate the intervention effects of Duhuo Jisheng Decoction(DHJSD)on this model.Methods After 7 d of adaptive feeding,24 male SD rats were randomized into blank group,model group,and DHJSD group.The blank group was only anesthetized by intraperitoneal injection of pentobarbital sodium,while the model group and DHJSD group were anesthetized by intraperitoneal injection of pentobarbital sodium for"partial resection of posterior column structure of the lumbar spine"and were fed in a cold and humid environment for six weeks.After two weeks of modeling,the blank group and model group were given an equal volume of distilled water by gavage,while the DHJSD group was administered DHJSD 18.6 g/(kg·d)by gavage for four consecutive weeks.The general condition,spontaneous pain behavior score,and neurological function score of rats in each group were compared.CT three-dimensional imaging was used to evaluate the morphological changes of the spinal physiological curvature,and the pathological section of intervertebral disc tissue was used to evaluate the severity of intervertebral disc nucleus pulposus lesions.Results After one week of modeling,compared with the blank group,the model group and the DHJSD group began to exhibit less movement,dull fur,drowsiness and curling up,slow response,loose stool,weight loss(P＜0.05),and increased spontaneous pain behavior score and neurological function score(P＜0.05).After three weeks of administration,compared with the model group,the pain behavior score and neurological function score of the DHJSD group decreased(P＜0.05).After four weeks of administration,compared with the model group,the spinal CT three-dimensional imaging showed no significant morphological degeneration in the DHJSD group,and the pathological section of intervertebral disc tissue also showed no obvious intervertebral disc nucleus pulposus lesions in the DHJSD group.Conclusion The method of"partial resection of posterior column structure of the lumbar spine+feeding in a cold and humid environment"can successfully construct the SD rat model of low back pain due to cold dampness stagnation,and DHJSD has good intervention effects on this model.
lower back paincold dampness stagnationDuhuo Jisheng Decoctionanimal modelintervertebral disc de-generation
万方数据
维普
