Objective To study Buxue Yimu Pill(BXYMP)treating mice with qi-blood deficiency based on hair metabolomics.Methods A mouse model of qi-blood deficiency was established by intraperitoneal injection of cyclophosphamide(CTX)and orbital bloodletting.After modeling,the animals were divided into model group,as well as low-and high-dose BXYMP groups which were intragastrically treated with BXYMP at doses of 1.82 and 7.28 g·kg-1 respectively for 10 consecutive days.Additionally,a normal group was set up for comparison.Gas chromatography time-of-flight mass spectrometry(GC-TOF-MS)was used to analyze the hair of mice from each experimental group.Principal component analysis(PCA)and orthogonal partial least-squares discriminant analysis(OPLS-DA)were employed to discover and identify the differential metabolites in the mice with qi-blood deficiency after the treatment of BXYMP.Results Five differential metabolites in the hair were associated with CTX-induced immunodeficiency of qi-blood deficiency pattern,mainly involving tyrosine metabolism and primary bile acid biosynthesis;after treatment with BXYMP,the metabolic profile approached that of the normal group,with 11 metabolites involved in three metabolic pathways.It was speculated that the therapeutic effect of BXYMP on qi-blood deficiency pattern may be related to the regulation of amino acid metabolism.Conclusion This study explains the TCM pattern of qi-blood deficiency and the intervention of BXYMP on it in ways of the mechanisms of amino acid metabolism and bile acid metabolism from the perspective of hair metabolomics.It partially reveals the mechanism of action of BXYMP in supplementing qi and tonifying blood,providing a certain theoretical basis for its clinical application.
关键词
代谢组学/补血益母丸/气血双虚/气质联用/毛发代谢
Key words
metabolomics/Buxue Yimu Pill/qi-blood deficiency/gas chromatography time-of-flight mass spectrometry/hair metabolism
维普
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