Objective To study the in vivo and in vitro anti-inflammatory activity,as well as isolation and identification of total saponins from Achyranthes aspera L.,providing experimental evidence for its treatment of inflammation.Methods The total saponins of Achyranthes aspera L.were purified and prepared by ODS-AQ;after modeling of ammonia-induced acute pharyngitis,rats were treated with corresponding drugs by gavage to evaluate the anti-inflammatory activities of the extract and total saponins of Achyranthes aspera L;column chromatography,nuclear magnetic resonance spectroscopy(NMR),mass spectrometry(MS),and other techniques were used to isolate,purify,and identify the main compounds in the total saponins of Achyranthes aspera L.;a lipopolysaccharide-induced macrophage inflammation model was established to evaluate the anti-inflammatory activity of various saponin monomers.Results The prepared total saponins of Achyranthes aspera L.reached a content of 81.8％;in the acute pharyngitis rat model,the total saponins and aqueous extract of Achyranthes aspera L.showed good anti-inflammatory effects,significantly reducing the serum levels of IL-6,IL-1β,and TNF-α;six oleanane-type triterpenoid saponins were identified as chikusetsusaponin Ⅳ a(1),chikusetsusaponin Ⅳ a butyl ester(2),calendulaside E(3),calendulaside E butyl ester(4),chikusetsusaponin I(5),and chikusetsusaponin V(6).In vitro inflammation model indicated that the isolated triterpenoid saponin monomers all exhibited varying degrees of anti-inflammatory effects.Conclusion This paper systematically demonstrates for the first time that saponin components are the pharmacological material basis for the anti-inflammatory activity of Achyranthes aspera L..Compounds 2 and 4 with anti-inflammatory effects are ester first isolated from Achyranthes plants
关键词
粗毛牛膝/抗炎/咽炎/三萜皂苷/化学成分/分离鉴定
Key words
Achyranthes aspera L./antiinflammation/pharyngitis/triterpenoid saponin/chemical composition/isolation and identification
维普
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