Effects of Back-Shu functional area pushing manipulation on gut microbiota and tryptophan metabolism in rats with chronic fatigue syndrome
Objective To explore the relief of symptoms in rats with chronic fatigue syndrome(CFS)through the Back-Shu functional area pushing manipulation,and its effects on their gut microbiota and tryptophan metabolism.Methods Twenty-four SPF-grade SD rats were selected and randomized into blank group(n=8)and modeling group(n=16).The modeling group was used to prepare the CFS model for 21 d,which,after the successful modeling,was randomly subdivided into model group and pushing manipulation(PM)group,with eight rats in each group.The intervention of pushing manipulation lasted for 14 d.After conducting the open-field test and exhaustive swimming experiment,the rat serum and colon were collected.Enzyme-linked immunosorbent assay(ELISA)was used to examine serum tryptophan(TRP)and kynurenine(KYN),and colonic indoleamine 2,3-dioxygenase(IDO).Fecal samples from the intestines were collected and subjected to 16s rRNA sequencing for the analysis of gut microbiota structure and diversity.Results Compared with the blank group,the model group showed significant reductions in the total distance travelled,the number of grid crossing,the time and distance travelled in the central area,the number of entries into the central area,and the swimming time to exhaustion(P＜0.01);the PM group showed increases in all the aforementioned parameters compared with the model group(P＜0.05).At the phylum level,compared with the blank group,the model group showed a decrease in the proportions of Firmicutes,Bacteroidetes and Verrucomicrobia,while a significant increase in those of Proteobacteria and Acidobacteria;compared with the model group,the PM group showed a decrease in the proportions of Firmicutes and Acidobacteria,while an increase in those of Bacteroidetes and Proteobacteria.At the genus level,compared with the blank group,the model group showed a significant decrease in the proportions of Romboutsia and Ruminococcaceae UCG-005(UCG-005),while a significant increase in those of Lactobacillus and Bifidobacterium;compared with the model group,the PM group showed a decrease in the proportions of Lactobacillus and Dubosiella,while an increase in those of Romboutsia and UCG-005.Alpha and Beta diversity analysis showed that,compared with the blank group,the model group's microbial richness and diversity were decreased;compared with the model group,the PM group showed a significant increase in microbial richness and diversity;however,the differences between the groups were not statistically significant(P＞0.05).LEfSe analysis showed that compared with the blank group,the dominant species in the model group were mainly clustered in the order,family,and genus levels,while the dominant species in the PM group were mainly clustered in the family and genus levels.Compared with the blank group,the model group had a significant decrease in serum TRP content(P＜0.01)and a significant increase in serum KYN and colonic IDO content(P＜0.01);compared with the model group,the PM group had a significant increase in serum TRP content(P＜0.01)and a decrease in serum KYN and colonic IDO content(P＜0.05).Correlation analysis revealed that the bacterial genera with greater correlation with TRP included Lachnospiraceae_NK4A 136_group,Lactobacillus,and Romboutsia(P＜0.05),those with greater correlation with KYN included Bifidobacterium05,Lachnospiraceae_NK4A136_group,and UCG-005(P＜0.05 or P＜0.01),and those with greater correlation with IDO included Allobaculum,Lachnospiraceae_NK4A136_group,and Romboutsia(P＜0.05 or P＜0.01).Conclusion The Back-Shu functional area pushing manipulation can significantly relieve fatigue and anxiety symptoms in CFS rats,regulate the content of microbial metabolites TRP,KYN,and colonic IDO,and has a certain regulating effect on the structure and diversity of the gut microbiota.Moreover,some bacterial genera have a correlation with TRP,KYN,and IDO content.
万方数据
维普
