Immune infiltration analysis of cuproptosis-related genes in osteoporosis and prediction of potential Chinese medicines
Objective To analyze the role of cuproptosis-related genes in immune infiltration of osteoporosis(OP),so as to explore the immune cells,immune function,biomarkers,and potential therapeutic Chinese medicines related to OP.Methods The OP datasets were retrieved and downloaded from the GEO database,and they were standardized and merged after eliminating batch effects.After extracting the relevant cuproptosis-related genes from the datasets,the immune infiltration analysis was carried out and the risk model was constructed to perform enrichment analysis on the cuproptosis-related genes and to predict Chinese medicines with potential therapeutic effects.Results(1)Eighteen cuproptosis-related genes were screened from the merged datasets of GSE13850,GSE56116,GSE56815,and GSE230665;(2)dendritic cells,B cells,CD8+T cells,and other cells accounted for a relatively high proportion in cell infiltration,and immune cell functions mainly manifested as antigen presentation co-suppression,type Ⅰ interferon response,type Ⅱ interferon response,and others;(3)compared with the healthy control group,macrophages and immature dendritic cells were highly expressed in the OP group,while follicular helper T cells were significantly expressed in the healthy control group;(4)thirteen cuproptosis-related genes including SLC31A1 were associated with OP immune infiltration,among which SLC31A1 was the most likely risk factor for OP;(5)the progress of OP involved biological processes such as acetyl-CoA biosynthesis and metabolism,which were related to fatty acid metabolism,tricarboxylic acid cycle,and other pathways;(6)a total of ten important Chinese medicines,including fish bladder glue,were screened out.Most of them are warm and neutral in property,sweet in flavour,and access the kidney and spleen meridians.And their effects are mostly supplementing qi,strengthening the spleen,tonifying the kidney,circulating blood,moving qi,and alleviating pain.Conclusion Cuproptosis-related genes may be involved in the progression of OP by intervening with immune cells and functions.Those such as SLC31A1 may help elucidate the pathogenesis of OP and become potential biological markers and therapeutic targets.Moreover,Chinese medicines such as fish bladder glue may be the source of potential molecular drugs for OP prevention and treatment.
cuproptosisosteoporosisimmune infiltrationsingle-sample gene set enrichment analysisChinese medicines prediction
万方数据
维普
