Toxicity reduction mechanism of Cangerzi(Xanthii Fructus)stir-fried with sand based on HMGB1 inflammatory pathway
Objective To study the toxicity reduction mechanism of Cangerzi(Xanthii Fructus)stir-fried with sand based on high mobility group box protein B1(HMGB1)inflammatory pathway,and to provide experimental basis for the toxicity reducing mechanism of its processing.Methods KM male mice were randomized into normal,raw Cangerzi(Xanthii Fructus),and Cangerzi(Xanthii Fructus)stir-fried with sand groups.The raw Cangerzi(Xanthii Fructus)and Cangerzi(Xanthii Fructus)stir-fried with sand groups were given corresponding drugs by gavage,respectively,the normal group was administered with the same amount of normal saline by gavage,for 14 d continually.The serum alanine transaminase(ALT)and aspartate transaminase(AST)of mice were measured 1 h after administration on the 14th day.The body mass and liver coefficient of mice were tested.The liver histopathology of mice was observed after HE staining.The serum levels of inflammatory factors HMGB1,interleukin-1β(IL-1β),interleukin-2(IL-2),and tumor necrosis factor-α(TNF-α)of mice were determined by ELISA.Western blot and RT-qPCR were performed to test protein and mRNA expression levels of HMGB1,nuclear factor-κB(NF-κB),and Toll-like receptor 4(TLR4)in liver tissues of mice,respectively.Results After the administration,compared with the normal group,the serum values of AST and ALT of the raw Cangerzi(Xanthii Fructus)and Cangerzi(Xanthii Fructus)stir-fried with sand groups increased(P<0.05),the body mass decreased(P<0.05),the pathological structure of the liver was damaged,the liver index increased(P<0.05),and the serum levels of HMGB1,IL-1β,IL-2,and TNF-α were all higher(P<0.01).Meanwhile,the values of Cangerzi(Xanthii Fructus)stir-fried with sand group were lower than those of raw Cangerzi(Xanthii Fructus)group(P<0.01).Westen blot and RT-qPCR showed that the protein and mRNA expressions of HMGB1,TLR4,and NF-κB in liver tissue of the medication groups were all significantly higher(P<0.05),which were more obvious in the raw Cangerzi(Xanthii Fructus)group than those in the Cangerzi(Xanthii Fructus)stir-fried with sand group(P<0.05).Conclusion Cangerzi(Xanthii Fructus)has certain hepatotoxicity,and its toxicity was significantly reduced after being stir-fried with sand.The toxicity reduction mechanism may be related to the regulation of HMGB1-TLR4/(NF-κB)inflammatory pathway.
Cangerzi(Xanthii Fructus)method of stir-frying with sandhepatotoxicityhigh mobility group box protein B1 inflammatory pathwaybiological mechanism
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