Regulatory mechanism of Achyranthis bidentatae Radix extract-containing serum on neuronal oxidative stress and apoptosis induced by Aβ1-42
Objective To explore the effects and mechanism of Achyranthis bidentatae Radix extract-containing serum on neuronal oxidative stress and apoptosis induced by amyloid β-protein1-42(Aβ1-42).Methods Rat hippocampal neurons were cultured in vitro and divided into control group,model group(Aβ1-42 induced nerves),low-,medium-,and high-dose Achyranthis bidentatae Radix groups(15,30,45 μg/mL Achyranthis bidentatae Radix extract-containing serum on Aβ1-42-induced nerves),si-ZBTB20-AS1 group[Aβ1-42 induced neurons transfected with antisense RNA of zinc finger and BTB domain protein 20 small interfering RNA]and si-NC group(Aβ1-42 induced neurons transfected with disorderly meaningless negative sequence).Content of superoxide dismutase(SOD)and malondialdehyde(MDA)were determined by ELISA;CCK-8 method was used to check the cell survival rate;flow cytometry was used to test the apoptosis rate;RT-qPCR was used to check the expression level of ZBTB20-AS1;Western blot method was used to measure the expression levels of cell proliferation antigen Ki67 and cysteine aspartic acid-specific protease-3(Caspase-3)protein.Results Compared with control group,SOD content,cell survival rate,and the protein expression of Ki67 in model group were lower(P<0.05),MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were higher(P<0.05).Compared with the model group,SOD content,cell survival rate,and the protein expression of Ki67 in the middle-and high-dose Achyranthis bidentatae Radix extract groups were higher(P<0.05),and MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were lower(P<0.05).Compared with the si-NC group,SOD content,cell survival rate,and the protein expression of Ki67 in the si-ZBTB20-AS1 group were higher(P<0.05),and MDA content,apoptosis rate,the expression of BTB20-AS1,and the protein expression of Caspase-3 were lower(P<0.05).Conclusion Achyranthis bidentatae Radix extract-containing serum may inhibit Aβ1-42 induced oxidative stress and apoptosis of hippocampal neurons by down-regulating the expression of ZBTB20-AS1.
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