Mechanism of action of Cyclocarya paliurus in treating diabetic rats with nonalcohol fatty liver disease based on UPLC-QE-MS metabolomics
Objective The application of non-targeted hepatic metabolomics and animal experiments was utilized to investigate the mechanisms by which Cyclocarya paliurus ameliorates diabetic fatty liver.Methods SD rats were randomly assigned to control,model,and Cyclocarya paliurus group(0.9 g·kg-1),with six rats in each group.An animal model of diabetes mellitus combined with non-alcoholic fatty liver disease was established,and tissue samples were collected after four weeks of continuous gavage.Fasting blood sugar(FBS),alanine aminotransferase(ALT),aspartate aminotransferase(AST),and triglyceride(TG)levels were measured using an automatic biochemical analyzer.The levels of glutathione(GSH),malondialdehyde(MDA),and superoxide dismutase(SOD)in liver tissues were quantified using ELISA.HE was employed to observe liver histopathology and morphology,while Oil Red O was used to assess liver lipid accumulation.Periodic acid-Schiff(PAS)staining was conducted to evaluate the distribution of liver glycogen and other polysaccharides.Additionally,differential metabolic pathways and related targets were analyzed through hepatic metabolomics.Results Compared to the model group,Cyclocarya paliurus significantly increased the levels of GSH and SOD in the liver tissues of the model rats,while also significantly decreasing the levels of FBS,TG,ALT,and AST(P＜0.001),and reducing hepatic lipid deposition.Metabolomics analysis identified eight biomarkers and one major metabolic pathway.Conclusions Cyclocarya paliurus attenuates hepatic lipid deposition and blood glucose levels in rats with diabetes mellitus combined with fatty liver,potentially through the regulation of butyric acid metabolism.
万方数据
维普
