Effects of Rabdosia Rubescens and oridonin on the inflammatory model of rheumatoid arthritis with blood stasis pattern in mice
Objective To study the effects of Chinese medicine Rabdosia Rubescens(RR)and its main active ingredient oridonin(ORI)on inflammation in a mouse model of rheumatoid arthritis(RA)with blood stasis pattern,and provide new therapeutic drugs for the treatment of this disease.Methods The C57BL/6 female mice were randomized into Control group,Model group,low concentration RR(L-RR)group,high concentration RR(H-RR)group,low concentration ORI(L-ORI)group,and high concentration ORI(H-ORI)group,with six mice in each group.Except for Control group,the other five groups were injected with 10 μl of emulsified complete Freund's adjuvant into the foot pads of bilateral hind limbs once.Two hours after the subcutaneous injection of adrenaline hydrochloride 0.1 mg/kg on the back,they were placed in cold water at 4℃ for five minutes,once a day,for seven consecutive days.L-RR and H-RR groups were treated with RR solution at the doses of 240 mg/(kg·d)and 480 mg/(kg·d)respectively by gavage,while L-ORI and H-ORI groups were intraperitoneally injected with ORI solution at the doses of 20 mg/(kg·d)and 40 mg/(kg·d)respectively,once a day,for 14 consecutive days.The thickness of the hind paws of mice was examined every 3 days.After 14-day treatment,the body mass was measured and the arthritis index score was evaluated.Besides,ELISA was used to check the conc-entrations of vascular endothelial growth factor A(VEGFA),tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1 β),and interleukin-6(IL-6)in the toes of mice,while HE staining and safranin-fast green staining to assess the degree of the ankle joint tissue damage.Results There was no statistically significant difference in body mass among the six groups(P＞0.05).Compared with Control group,Model group showed joint swelling,infiltration of inflammatory cells in the joints,synovial hyperplasia,and cartilage destruction with increases in the thickness of the hind paws,arthritis index score,as well as the concentrations of TNF-α,IL-6,IL-1β,and VEGFA(P＜0.05).Compared with Model group,L-RR,H-RR,L-ORI,and H-ORI groups showed alleviated joint swelling;H-RR,L-ORI,and H-ORI groups had decreased hind paw thickness after 22 days(P＜0.05);H-RR and H-ORI groups had reduced arthritis index scores(P＜0.05);H-RR,L-ORI,and H-ORI groups had lower concentrations of TNF-α,IL-6,and IL-1 β(P＜0.05);H-RR,L-ORI,and H-ORI groups had lower VEGFA concentration(P＜0.05);L-RR,H-RR,L-ORI,and H-ORI groups all had reduced inflammatory cell infiltration,synovial hyperplasia,and cartilage destruction in the ankle joint tissue.Conclusion For a mouse model of RA with blood stasis pattern,RR and ORI can alleviate the joint swelling,decrease the hind paw thickness,lower the arthritis index scores and the concentrations of inflammatory factors including TNF-α,IL-6,IL-1β,and VEGFA,and reduce the inflammatory cell infiltration,synovial hyperplasia,and cartilage destruction in the ankle joint tissue.This indicates that RR and its main active ingredient ORI have therapeutic effects on the inflammatory model of RA with blood stasis pattern,and VEGFA may serve as an indicator for evaluating the efficacy of treatments for RA with blood stasis pattern.
万方数据
维普
