Effects of Danggui Shaoyao San on the intestinal flora of db/db mice with diabetic nephropathy
Objective To study the effects of Danggui Shaoyao San(DSS)on the intestinal flora of mice with diabetic nephropathy(DN).Methods Thirty 8-week-old male db/db mice and six 8-week-old male db/m mice were selected.Fasting blood glucose(FBG)and urinary albumin to creatinine ratio(UACR)were measured,and the qualified db/db mice were randomized into five groups:model(MOD)group(distilled water 0.1 mL/10 g),dapagliflozin(DAPA)group(dapagliflozin tablets 1.3 mg/kg),low-dose DSS(DSS-L)group(8.39 g/kg),medium-dose DSS(DSS-M)group(16.77 g/kg),and high-dose DSS(DSS-H)group(33.54 g/kg),with six mice in each group.The six db/m mice served as the blank control(CON)group(distilled water 0.1 mL/10 g).Then FBG and insulin tolerance in mice were measured.Blood urea nitrogen(BUN)and serum creatinine(Scr)were measured by ELISA,and UACR was detected by fully automatic biochemical analyzer.Samples of the mouse renal and small intestinal tissues were taken for HE staining,and feces in the mouse colon was selected for 16S rRNA sequencing.Results Compared with the CON group,the MOD group had lower glucose tolerance and higher levels of FBG,BUN,Scr,and UACR(P＜0.01).Compared with the MOD group,the DSS group exhibited increased glucose tolerance(P＜0.01),decreased levels of FBG,BUN,Scr,and UACR(P＜0.01),and reduced pathological damage to the kidney and small intestine.The study on the intestinal flora diversity show that compared with the CON group,the MOD group had a lower diversity of intestinal flora.Compared with the MOD group,the flora differences between the DSS-M group and the CON group were smaller.In terms of the species composition of the flora,the relative abundance of Akkermansia muciniphila in the DSS-M group of mice was significantly higher(P＜0.01).Conclusions DSS can significantly increase the abundance of Akkermansia muciniphila in the intestines of db/db mice with DN,which may be one of the mechanisms by which it improves DN.
diabetic nephropathyDanggui Shaoyao Sanintestinal floracirculating blood and draining water retentiondiabetes16S rRNAChinese medicine
万方数据
维普
