Analysis of the mechanism of action of Qingfei Decoction's anti-pneumonia activity by UPLC-Q-Exactive Orbitrap-MS combined with network pharmacology
Objective To explore the mechanism of action of Qingfei Decoction's anti-pneumonia activity using UPLC-Q-Excative Orbitrap-MS and network pharmacology.Methods The major chemical components of Qingfei Decoction were analyzed and identified by UPLC-Q-Excative Orbitrap-MS.Targets of the identified components were collected using Swisstarget pneumonia-related targets were retrieved from OMIM,DrugBank,and GeneCards databases.Common targets between the identified components of Qingfei Decoction and pneumonia were screened by taking their intersection Protein-protein interaction(PPI)network construction and GO/WikiPathways enrichment analyses were performed to identify key signaling pathways involved in Qingfei Decoction's treatment of pneumonia.Results A total of 142 active ingredients were identified from Qingfei Decoction by UPLC-Q-Excative Orbitrap-MS,yielding 200 intersecting targets between the ingredients and pneumonia.Network construction identified 44 potential effective components and 131 key pneumonia-related targets.KEGG pathway enrichment analysis indicated that Qingfei Decoction exerted its anti-pneumonia effects through multiple signaling pathways,including the MAPK3 signaling pathway,EGFR signaling pathway,and LCK signaling pathway,as well as multiple targets such as interleukin-5(IL-5),interleukin-2(IL-2),and thymic stromal lymphopoietin(TSLP).Conclusion The active ingredients in Qingfei Decoction can exert therapeutic effects against pneumonia via multi-pathway and multi-target mechanisms,including immune regulation,anti-inflammatory action,modulation of apoptosis,antiviral effects,and mitigation of pulmonary fibrosis.
UPLC-Q-Excative Orbitrap-MSQingfei Decoctionpneumonianetwork pharmacologymechanism of action
万方数据
维普
