Chemical constituents and antioxidant activity of Potentilla freyniana Bornm.
Objective To study the chemical constituents of the dichloromethane and ethyl acetate fractions of the roots of Potentilla freyniana Bornm.and to screen for their antioxidant active components.Methods The dichloromethane and ethyl acetate fractions of the ethanol extract were separated and purified using silica gel chromatography,gel chromatography,and semi-preparative HPLC.The structures of the isolated compounds were identified by NMR and MS.The antioxidant activities of the flavonoids 7 and 9 were evaluated through hydroxyl radical scavenging assay,DPPH radical scavenging assay,and ABTS radical scavenging assay.Results Seventeen compounds were isolated from P.freyniana and identified as daucosterol(1),β-sitosterol(2),7-keto-β-sitosterol(3),naringenin(4),carthamidin(5),dihydrokaempferol(6),eriodictyol(7),quercetin(8),catechin(9),thunberginol C(10),rhododenol(11),4-(4-carboxy-2-methoxyphenoxy)-3,5-dimethoxybenzoic acid(12),butylene phthalate(13),vanillic acid(14),protocatechuic acid(15),phydroxybenzoic acid(16),and lindleyin(17).Among them,compound 4 was identified from this plant for the first time,and compounds 3,10,12,13,and 14 were separated from this genus for the first time.When the concentration of compound 7 exceeds 5 mmol/L and the concentration of compound 9 exceeds 2 mmol/L,the scavenging rate of hydroxyl radicals exceeds 90%,and the scavenging rates of DPPH radicals and ABTS radicals exceed 95%.The antioxidant level is similar to that of VC,indicating significant antioxidant performance.Conclusion Compounds 7 and 9 exhibited certain potential in antioxidant and anti-inflammatory activities.This study provides some experimental basis and scientific references for the chemical composition and biological activity of Potentilla freyniana Bornm.,promoting the further development and utilization of its active components.
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